[Histonet] RE: Fragmented Biopsy Samples

[Nails, Felton]

Try using histoscreen cassettes and that will eliminate the need for wrapping the tissue.
If still insist on wrapping, ensure that they are dipping the tissue paper in formalin prior to putting the tissue on the paper.
Also at embedding, if the tissue is stuck to the paper, take a blade and put it against the tissue and gently push to loosen the tissue, repeat this the length of the tissue. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, August 30, 2012 10:41 AM
To: Willis, Donna G.; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Fragmented Biopsy Samples

Donna, try the new SecureSette from ThermoFisher. They enclose the sample at grossing and do not require manipulation at embedding - just add wax and cool. Very interesting product. We tried them and they work well for some samples. However, they only accommodate straight needle bx of 2cm or less. Our prostate cores were 2.5 cm at times so it didn't quite fit.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken <@t> ucsfmedctr.org




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Thursday, August 30, 2012 8:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fragmented Biopsy Samples

Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding.  Don't want to use sponges.

Thanks,

Donna Willis, HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.willis <@t> baylorhealth.edu

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