[Histonet] DAPI staining

[Mark Tarango]

Hi Carol,

Does this person put them in a freezer for 20 mins or so after DAPI
staining?  Someone once told me this helps with smearing.  I'm not really
sure if its true, but couldn't hurt to try it.

Mark

On Thursday, August 30, 2012, Barone, Carol wrote:

> Someone sent me a question regarding DAPI staining ( I generally  use
> Hoechst )...but anyway, they are having smearing of nuclear contents. I
> would guess that is from rupturing of the nuclear membrane....over
> fixation? Over air drying? They are fixing 10 mins in cold acetone and 10
> mins to air dry. What do the experts out there say? This could be a
> learning moment for me , as well.
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