[Histonet] folds in tissue

Lynette Pavelich LPaveli1 <@t> hurleymc.com
Fri Aug 24 09:08:26 CDT 2012

I know that animal tissue is very different than human and we deal only with human. But we share the same issues here. We have found that floating the tissue in a room temperature dish of 30% alcohol and then transferring them to the water bath has helped us.
Interesting to hear what others are doing!! I'll have to try the room temp water!


Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966

From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs <@t> yahoo.com]
Sent: Friday, August 24, 2012 9:00 AM
To: Jennifer Sipes; Histonet
Subject: Re: [Histonet] folds in tissue

Try floating your sections on room temperature (in a shallow dish) water then carefully, on a slide, transfer to the waterbath. Sections will spread better when they hit the warm water.
Good luck!

Sheila Haas
Laboratory Manager
MicroPath Laboratories, Inc.

From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, August 24, 2012 8:50 AM
Subject: [Histonet] folds in tissue

Good morning/afternoon/evening Histonetters!

I'm having a problem with my tissue folding.  It's tiny folds in mouse tissue that are too little to see without the use of a microscope.  I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope.

I'm using distilled water in my water bath and it's set to about 40 degrees Celcius.

All help is appreciated!  Thank you all so much for your time!


K. Sipes, ALAT
Sr. Laboratory Technician
Johns Hopkins University
Ross 933
720 Rutland Avenue
Baltimore, MD  21205
phone:     410-614-0131
fax:         410-955-9677
cell:         443-631-6361
e-mail:  jsipes1 <@t> jhmi.edu
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