[Histonet] folds in tissue

[Rene J Buesa]

For this difficult tissue, and for any other for that matter, add to your regular round water bath at 45ºC, 2 mL of liquid dish washer soap. The soap will reduce the surface tension of the water and will allow the sections to extend completely, eliminating any folds.
René J.


________________________________
From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, August 24, 2012 8:50 AM
Subject: [Histonet] folds in tissue

Good morning/afternoon/evening Histonetters!
 
I'm having a problem with my tissue folding.  It's tiny folds in mouse tissue that are too little to see without the use of a microscope.  I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope.
 
I'm using distilled water in my water bath and it's set to about 40 degrees Celcius.
 
All help is appreciated!  Thank you all so much for your time!
 
Regards,
 
Jen 
Jennifer
K. Sipes, ALAT 
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 933 
720 Rutland Avenue 
Baltimore, MD  21205 
phone:     410-614-0131 
fax:         410-955-9677 
cell:         443-631-6361 
e-mail:  jsipes1 <@t> jhmi.edu
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