[Histonet] RE: Eosin

[Bernice Frederick]

We add our own 1% phloxine in 95% alcohol.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, August 09, 2012 10:28 AM
To: Hannen, Valerie; histonet
Subject: [Histonet] RE: Eosin

Buy one with Phloxine in it.  It will make it more red and stronger...

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Thursday, August 09, 2012 10:23 AM
To: histonet
Subject: [Histonet] Eosin

Hi Folks...I am hoping you all give me a little help.  Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one

day to the next...one day it looks great...the next it is weak!!

I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the  alcohols after Eosin are the proper concentrations....3)

 reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing

 steps. 5) I have checked the pH of the water as well.

Any help and suggestions would greatly appreciated!!

Thanks Gang!!

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com







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