[Histonet] RE: Eosin

[Lynette Pavelich]

I can still hear one of our pathologist's coming down the hall, loudly complaining that "SOMETHING'S WRONG WITH THE EOSIN!!!!!!"

We added new, we changed to completely new, we played with the times.......all to no avail.......

Then we changed the hematoxylin..............down the hall she came............"YOU FIXED THE EOSIN!!! THANK YOU!!"

Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sarah Dysart [sdysart <@t> mirnarx.com]
Sent: Thursday, August 09, 2012 11:27 AM
To: Hannen, Valerie; histonet
Subject: [Histonet] RE: Eosin

Buy one with Phloxine in it.  It will make it more red and stronger...

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Thursday, August 09, 2012 10:23 AM
To: histonet
Subject: [Histonet] Eosin

Hi Folks...I am hoping you all give me a little help.  Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one

day to the next...one day it looks great...the next it is weak!!

I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the  alcohols after Eosin are the proper concentrations....3)

 reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing

 steps. 5) I have checked the pH of the water as well.

Any help and suggestions would greatly appreciated!!

Thanks Gang!!

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com







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