[Histonet] Eosin

[Jennifer Campbell]

What clearing agent are you using?

If it is a xylene substitute then you might want to rotate or change the
alcohols after eosin after a few racks of slides.

We use ProPar on our stainer and rotate our alcohols after eosin after
every 3 racks that have gone through.



On Thu, Aug 9, 2012 at 11:23 AM, Hannen, Valerie <
Valerie.Hannen <@t> parrishmed.com> wrote:

> Hi Folks...I am hoping you all give me a little help.  Our Pathologists
> are complaining about our Eosin on the H&E's being weak. The funny thing
> is, is that it can go one
>
> day to the next...one day it looks great...the next it is weak!!
>
> I have already done some experimenting with...1) time tissue spends in
> Eosin.... 2) making sure that the  alcohols after Eosin are the proper
> concentrations....3)
>
>  reducing the time that the tissues spends in the alcohols atfer Eosin...
> I have even gone as far as 4) increasing the rinse time in water after the
> decolorizing and bluing
>
>  steps. 5) I have checked the pH of the water as well.
>
> Any help and suggestions would greatly appreciated!!
>
> Thanks Gang!!
>
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.hannen <@t> parrishmed.com
>
>
>
>
>
>
>
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-- 
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137


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