[Histonet] Eosin

[Geoff McAuliffe]

Add a few drops of glacial acetic acid.

Geoff

On 8/9/2012 11:23 AM, Hannen, Valerie wrote:
> Hi Folks...I am hoping you all give me a little help.  Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one
>
> day to the next...one day it looks great...the next it is weak!!
>
> I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the  alcohols after Eosin are the proper concentrations....3)
>
>   reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing
>
>   steps. 5) I have checked the pH of the water as well.
>
> Any help and suggestions would greatly appreciated!!
>
> Thanks Gang!!
>
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.hannen <@t> parrishmed.com
>
>
>
>
>
>
>
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-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
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