[Histonet] Ki67 antibody to stain Mouse tissue

[Colleen Forster]

FYI: That is what the data sheet says because they didn't test it but 
many of us have and it works beautifully!

It should stain mostly in the bottom of the crypts where the new cells 
emerge from but you will have occasional cells towards the top of the 
crypt. It should look much like it does in human gut and yes, I have 
done it on most major organs of the mouse.

Colleen Forster
U of MN



On 8/8/2012 10:38 AM, Eva Permaul wrote:
> Just to clarify. Are you using it on mouse tissues? I am only asking
> because the Thermo Scientific/Labvision/Neomarkers data sheet says it has
> only been verified on Human tissues.
> Secondly had anyone used it on mouse gut. Does it stain in the bottom of
> the crypts or at the top? I am asking because I have also tried a different
> antibody from Leica but got staining at the top of the crypts not at the
> bottom.
>
> Thank you to everyone for all of your valuable suggestions and advice,
> Eva Permaul
> Georgetown University
>
> On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) <
> joost.bruijntjes <@t> tno.triskelion.nl> wrote:
>
>> Hi Eva
>>
>> I've used an antibody from Labvision /Neomarkers. It is a rabbit
>> monoclonal; catalog number: RM-9106.
>>
>> Joost
>>
>> -----Oorspronkelijk bericht-----
>> Van: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
>> histonet-bounces <@t> lists.utsouthwestern.edu] Namens Eva Permaul
>> Verzonden: maandag 6 augustus 2012 20:44
>> Aan: histonet
>> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue
>>
>> Hello,
>> I am looking for a Ki67 to stain mouse tissues. We have been using Dako
>> M2749 but just found out it has been discontinued. Could anyone suggest a
>> good alternative?
>> Thank you all for any help,
>> Eva Permaul
>> Georgetown University
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