[Histonet] RE: Histonet Digest, Vol 105, Issue 9
Webb, Dorothy L
Dorothy.L.Webb <@t> HealthPartners.Com
Wed Aug 8 10:42:42 CDT 2012
We make up a "marking Eosin" that we use on small bx before we place them in cassettes.
We do not use actual teabags, but the mesh bags from Thermo Fisher that are not hard to pull apart and we never have anything sticking I the corners. I do know what you are referring to as some of the mesh bags ot teabags on the market are not mad of as fine material and are hard to pull apart and allow for specimens to gather in the corners. Our mesh bags are used for all specimens the PA's can "pour" into the bags.
We also use the Obex papers for needle bx type specimens that can be laid out nicely on the paper and folded over the tissue once for optimal processing.
On tissue processors, we are new this year to the Peloris processor from Leica and love the versatility of two retorts and the great reagent management which allows for less waste and less maintenance tech time. I was always a Sakura VIP fan previous, still am, but cannot compare the two retort option and how LEAN it has mad our lab!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, August 08, 2012 9:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 105, Issue 9
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Today's Topics:
1. Re: opinion on heating slides prior to IHC (Teri Johnson)
2. Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with
2020 microtome? (Jennifer Johnson)
3. Re: Teabags (Bob Richmond)
4. RE: Re: Teabags (Sarah Dysart)
5. RE: Re: Teabags (Clouse, Rosanna)
6. Re: Re: Teabags (Paula Pierce)
7. RE: Re: Teabags (Shirley A. Powell)
8. tissue highlighting for visibility (contact <@t> histocare.com)
9. decalcification of premolar teeth (dog) (Alice Fraser)
10. National Society for Histotechnology Hard Tissue Forum Event
- Bethesda, MD on August 18, 2012!!! (Jack Ratliff)
11. help ! paraffin section (Megha Kumar)
12. Re: tissue highlighting for visibility (Lee & Peggy Wenk)
13. Re: Re: Teabags (Lee & Peggy Wenk)
14. RE: tissue highlighting for visibility (MaryK Mendell)
15. Tissue Processor (Heckford, Karen - SMMC-SF)
16. RE: tissue highlighting for visibility (Vanessa Perez)
17. Re: Tissue Processor (Rene J Buesa)
18. Re: tissue highlighting for visibility (Rene J Buesa)
19. RE: decalcification of premolar teeth (dog) (Rittman, Barry R)
20. Re: help ! paraffin section (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Tue, 7 Aug 2012 17:30:04 +0000
From: Teri Johnson <TJohnson <@t> gnf.org>
Subject: [Histonet] Re: opinion on heating slides prior to IHC
To: "jefthompson <@t> salud.unm.edu" <jefthompson <@t> salud.unm.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9F3CFEE76E51B64991C7485270890B400CDCB1B7 <@t> EX4.lj.gnf.org>
Content-Type: text/plain; charset="us-ascii"
Dear J,
I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process.
I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it.
As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to say they might have had to try multiple antibodies to find one that would work under those conditions?
The only way to know if heat or other conditions will negatively affect your target protein is to test it empirically.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
------------------------------
Message: 2
Date: Tue, 7 Aug 2012 12:11:53 -0700
From: Jennifer Johnson <jjohnson <@t> scripps.edu>
Subject: [Histonet] Manual for Reichert-Jung/Leica Cryocut 1800
Cryostat with 2020 microtome?
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9B9F464463CB36488E8904CF8336917FB76BB5708D <@t> EXCH-CCR01.lj.ad.scripps.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Guys
Does anyone have a copy of the manual for the Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? Thanks in advance.
Jennifer L. Johnson, Ph.D.
Staff Scientist
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla, CA 92037
jjohnson <@t> scripps.edu
------------------------------
Message: 3
Date: Tue, 7 Aug 2012 15:22:57 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Teabags
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH7EiYQof1F8dK9sSww+Vny-u-CZZVE+Ohu68Mmk_Pk0PA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Susan Walzer notes >>I was also tired of digging bone marrow particles
and biopsies out of the stitching. Some people like [teabags] because
they can just dump tissue in them but they do not have to fight with
them when embedding. Biopsy cassettes can trap air and float. The best
all around product is Obex round papers. For people who like to dump
you can fold them into cones and use like filter paper. They are the
best thing for all around protection of small and friable tissue.<<
I'm not familiar with Obex round papers. See http://histowrap.com/ for
more information.
Bob Richmond
Samurai Pathologist
Asbury Place, a continuing care retirement community in Maryville TN,
about half an hour south of Knoxville (but I have no plans to retire!)
------------------------------
Message: 4
Date: Tue, 7 Aug 2012 19:27:10 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: RE: [Histonet] Re: Teabags
To: Bob Richmond <rsrichmond <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8A70A9B2ECDD084DACFE6C59FCF86D5018D4D4D5 <@t> BL2PRD0710MB363.namprd07.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
I use hair perm papers that I buy from a local beauty supply store. WAY cheaper, and work very well!
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, August 07, 2012 2:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Teabags
Susan Walzer notes >>I was also tired of digging bone marrow particles
and biopsies out of the stitching. Some people like [teabags] because
they can just dump tissue in them but they do not have to fight with
them when embedding. Biopsy cassettes can trap air and float. The best
all around product is Obex round papers. For people who like to dump
you can fold them into cones and use like filter paper. They are the
best thing for all around protection of small and friable tissue.<<
I'm not familiar with Obex round papers. See http://histowrap.com/ for
more information.
Bob Richmond
Samurai Pathologist
Asbury Place, a continuing care retirement community in Maryville TN,
about half an hour south of Knoxville (but I have no plans to retire!)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Tue, 7 Aug 2012 15:31:36 -0400
From: "Clouse, Rosanna" <rclouse <@t> wellspan.org>
Subject: RE: [Histonet] Re: Teabags
To: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9E4560B21D59954A9931FF35B7BCCDD4021A444967 <@t> EXCH01.wellspan.org>
Content-Type: text/plain; charset="us-ascii"
For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks.
Rosanna S. Clouse, SCT(ASCP)
Division Manager - Cytology
Gettysburg Hospital - Wellspan
Gettysburg, PA 17325
email-rclouse <@t> wellspan.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, August 07, 2012 3:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Teabags
Susan Walzer notes >>I was also tired of digging bone marrow particles
and biopsies out of the stitching. Some people like [teabags] because
they can just dump tissue in them but they do not have to fight with
them when embedding. Biopsy cassettes can trap air and float. The best
all around product is Obex round papers. For people who like to dump
you can fold them into cones and use like filter paper. They are the
best thing for all around protection of small and friable tissue.<<
I'm not familiar with Obex round papers. See http://histowrap.com/ for
more information.
Bob Richmond
Samurai Pathologist
Asbury Place, a continuing care retirement community in Maryville TN,
about half an hour south of Knoxville (but I have no plans to retire!)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 6
Date: Tue, 7 Aug 2012 12:33:43 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: Re: [Histonet] Re: Teabags
To: Sarah Dysart <sdysart <@t> mirnarx.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1344368023.96408.YahooMailNeo <@t> web5706.biz.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Ditto!
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
________________________________
From: Sarah Dysart <sdysart <@t> mirnarx.com>
To: Bob Richmond <rsrichmond <@t> gmail.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, August 7, 2012 2:27 PM
Subject: RE: [Histonet] Re: Teabags
I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well!
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas? 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, August 07, 2012 2:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Teabags
Susan Walzer notes >>I was also tired of digging bone marrow particles
and biopsies out of the stitching. Some people like [teabags] because
they can just dump tissue in them but they do not have to fight with
them when embedding. Biopsy cassettes can trap air and float. The best
all around product is Obex round papers. For people who like to dump
you can fold them into cones and use like filter paper. They are the
best thing for all around protection of small and friable tissue.<<
I'm not familiar with Obex round papers. See http://histowrap.com/ for
more information.
Bob Richmond
Samurai Pathologist
Asbury Place, a continuing care retirement community in Maryville TN,
about half an hour south of Knoxville (but I have no plans to retire!)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 7 Aug 2012 16:04:53 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] Re: Teabags
To: Paula Pierce <contact <@t> excaliburpathology.com>, Sarah Dysart
<sdysart <@t> mirnarx.com>, Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE24B4555B779 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="iso-8859-1"
Same here.
sp
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, August 07, 2012 3:34 PM
To: Sarah Dysart; Histonet
Subject: Re: [Histonet] Re: Teabags
Ditto!
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
________________________________
From: Sarah Dysart <sdysart <@t> mirnarx.com>
To: Bob Richmond <rsrichmond <@t> gmail.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, August 7, 2012 2:27 PM
Subject: RE: [Histonet] Re: Teabags
I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well!
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas? 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, August 07, 2012 2:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Teabags
Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<<
I'm not familiar with Obex round papers. See http://histowrap.com/ for more information.
Bob Richmond
Samurai Pathologist
Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 07 Aug 2012 18:10:25 -0400
From: contact <@t> histocare.com
Subject: [Histonet] tissue highlighting for visibility
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20120807181025.46opnxy2fok408sk <@t> mail.histocare.com>
Content-Type: text/plain; charset=UTF-8
Hello all,
Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it,
a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort.
I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ??What do some of you guys do?
www.HistoCare.com
Histology Staffing
------------------------------
Message: 9
Date: Wed, 8 Aug 2012 13:56:08 +1200
From: Alice Fraser <toxpathnz <@t> gmail.com>
Subject: [Histonet] decalcification of premolar teeth (dog)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAO8o5+w0isL=bHHLGHd+g7yM94eh8sddySx909JqyK6W79KmKQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear Members
I would be really interested to hear your advice on the currently preferred
procedure for decalcification of premolar teeth from dogs. Do laboratories
find the HCl/water solution or the formic acid solution or another solution
to be optimal for decal without obliterating the tissues to be evaluated? A
procedure/method would be hugely appreciated if poss.
Many thanks.
Alice
------------------------------
Message: 10
Date: Tue, 7 Aug 2012 23:12:35 -0400
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: [Histonet] National Society for Histotechnology Hard Tissue
Forum Event - Bethesda, MD on August 18, 2012!!!
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU167-W653253FF1A9A2D3FC99CBCAECD0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Greetings! The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You won't want to miss out on this opportunity to learn about and witness via "LIVE" demonstration all current forms of resin/plastics histology equipment. A special treat this year will be a first ever showing and demonstration in North America of a newly developed non-contact laser microtome (TissueSurgeon) to cut micron thin sections of a variety of tissue types!
7:30am - Registration Opens
8:00am ? Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique ? Jack L Ratliff
9:00am ? Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It ? Daniel S Perrien, Ph.D.
10:30am ? Refreshment Break
10:45am ? Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome ? Damien Laudier, BS, HTL(ASCP), QIHC
12:15pm ? Lunch On Your Own
1:15pm ? Ground Section Microtomy Techniques (Parts I & II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens ? (PART I) Joe Tabeling & Jack L Ratliff, BA; (PART II) Linda Durbin & Robert A Skinner
2:45pm ? Refreshment Break
3:00pm ? New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses ? Heiko Richter, Ph.D.
4:00pm ? Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy ? Nathanael H Reveal
You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th!
Best Regards,
Jack Ratliff
Chairman, Hard Tissue Committee - National Society for Histotechnology
------------------------------
Message: 11
Date: Wed, 8 Aug 2012 09:15:47 +0530
From: Megha Kumar <meghak <@t> g.clemson.edu>
Subject: [Histonet] help ! paraffin section
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAGnJi_Wt9Gfd08ghkU72M5iGvmV+n0_4OCSTh6yHjAg65HROXA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding. However, when i section, the tissue is torn although the rest of
the paraffin looks perfect. Please suggest why this is happening. Also,
sometimes the skin sections fall off the slides when I perform in situ
hybridization. Any ideas how to prevent this?
Please help! i am a beginner in histology and dont' know what to do!
regards
Megha
*
*
------------------------------
Message: 12
Date: Wed, 8 Aug 2012 05:33:45 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] tissue highlighting for visibility
To: <contact <@t> histocare.com>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <75FEA5CFE2B246ED8F5B9D00FD2282A9 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="utf-8";
reply-type=original
Drop of hematoxylin on the tissue, when put on the paper in the grossing
area. Use a syringe. Only a SMALL drop. Too much means there's extra blue
all over the paper, making it hard to see the blue tissue.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
The opinions expressed are mine, and do not reflect those of Beaumont
Hospital.
-----Original Message-----
From: contact <@t> histocare.com
Sent: Tuesday, August 07, 2012 6:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue highlighting for visibility
Hello all,
Earlier today I had a VERY tiny sample from the esophogus. When I say it was
tiny, it looked to be only a few microns in thickness. It was inside of, you
guessed it,
a teabag! :) But that wasn't the problem, as it was appropriate in this case
to be put in a teabag because of the size. When I pulled it out of the
cassette, I had to go over it very carefully to even find it. It's sad that
I know of a not insignificant number of people who wouldn't have taken the
time to find it and most likely have dispositioned it as not surviving
processing or no tissue found, but that is another issue. I'm sure the
patient would appreciate the extra effort.
I know of a few techniques to make tissue, and specifically tiny or fatty
tissue, more easily visible in cases like these. For example, I've seen
using a different colored wax or putting eosin in the alcohol during
processing. What do some of you guys do?
www.HistoCare.com
Histology Staffing
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 8 Aug 2012 05:36:19 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Re: Teabags
To: "Clouse, Rosanna" <rclouse <@t> wellspan.org>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BFA4DEBDB1CA47F993F82674F3E6AF11 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hint when using these - do NOT try to fold them up into a nice looking
square. Once processed and in paraffin, it is very difficult to find the
edge, to try to open back up.
Fold into a not nice to look at, off-set square that is slightly crumpled.
Much easier to find the edge.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
The opinions expressed are mine, and do not reflect on Beaumont Hospital.
-----Original Message-----
From: Clouse, Rosanna
Sent: Tuesday, August 07, 2012 3:31 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Teabags
For those of you who like lens paper and/or the Obex Histo Wrap, a very
inexpensive alternative is to visit any beauty store or visit
sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand
2.5" x 4" sheets. We have used them for years and they work really well for
cell blocks.
Rosanna S. Clouse, SCT(ASCP)
Division Manager - Cytology
Gettysburg Hospital - Wellspan
Gettysburg, PA 17325
email-rclouse <@t> wellspan.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, August 07, 2012 3:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Teabags
Susan Walzer notes >>I was also tired of digging bone marrow particles
and biopsies out of the stitching. Some people like [teabags] because
they can just dump tissue in them but they do not have to fight with
them when embedding. Biopsy cassettes can trap air and float. The best
all around product is Obex round papers. For people who like to dump
you can fold them into cones and use like filter paper. They are the
best thing for all around protection of small and friable tissue.<<
I'm not familiar with Obex round papers. See http://histowrap.com/ for
more information.
Bob Richmond
Samurai Pathologist
Asbury Place, a continuing care retirement community in Maryville TN,
about half an hour south of Knoxville (but I have no plans to retire!)
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Message: 14
Date: Wed, 8 Aug 2012 06:47:56 -0400
From: MaryK Mendell <kmendell <@t> goldbergmd.net>
Subject: RE: [Histonet] tissue highlighting for visibility
To: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>, "contact <@t> histocare.com"
<contact <@t> histocare.com>, "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<96138C8AB728814B9A576E4364EF84F9012BD954D328 <@t> EXMBX01.mmeprod.cbeyond>
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ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop
Kate Mendell
Histopathology/Lab Manager
HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA 01907
TEL: 781.595.0151
FAX: 781.592.6780
kmendell <@t> goldbergmd.net
www.cosmesticdermcenter.com
PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk <@t> sbcglobal.net]
Sent: Wednesday, August 08, 2012 5:33 AM
To: contact <@t> histocare.com; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] tissue highlighting for visibility
Drop of hematoxylin on the tissue, when put on the paper in the grossing
area. Use a syringe. Only a SMALL drop. Too much means there's extra blue
all over the paper, making it hard to see the blue tissue.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
The opinions expressed are mine, and do not reflect those of Beaumont
Hospital.
-----Original Message-----
From: contact <@t> histocare.com
Sent: Tuesday, August 07, 2012 6:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue highlighting for visibility
Hello all,
Earlier today I had a VERY tiny sample from the esophogus. When I say it was
tiny, it looked to be only a few microns in thickness. It was inside of, you
guessed it,
a teabag! :) But that wasn't the problem, as it was appropriate in this case
to be put in a teabag because of the size. When I pulled it out of the
cassette, I had to go over it very carefully to even find it. It's sad that
I know of a not insignificant number of people who wouldn't have taken the
time to find it and most likely have dispositioned it as not surviving
processing or no tissue found, but that is another issue. I'm sure the
patient would appreciate the extra effort.
I know of a few techniques to make tissue, and specifically tiny or fatty
tissue, more easily visible in cases like these. For example, I've seen
using a different colored wax or putting eosin in the alcohol during
processing. What do some of you guys do?
www.HistoCare.com
Histology Staffing
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 15
Date: Wed, 8 Aug 2012 05:11:13 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>
Subject: [Histonet] Tissue Processor
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CCC690F0F65F9348BBBF341DFF0D175A19E563B470 <@t> PHX-MSG-007-N1.chw.edu>
Content-Type: text/plain; charset="us-ascii"
I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help.
Cheers,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
------------------------------
Message: 16
Date: Wed, 8 Aug 2012 07:53:24 -0500
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: RE: [Histonet] tissue highlighting for visibility
To: MaryK Mendell <kmendell <@t> goldbergmd.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C80B5EE351AAD74DADAB70FABCB78D193BCA8DA5F9 <@t> exchange.pasa.local>
Content-Type: text/plain; charset="us-ascii"
We use microwave processing and we add hematoxylin to the absolute and eosin to the isopropyl....this also helps in keeping techs from accidently using isopropyl as absolute or vice-versa... when grosser cant find tissue in the container we put a drop of eosin and swirl and filter to try and find any material in the formalin container.
Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
210-892-3732
vperez <@t> pathreflab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of MaryK Mendell
Sent: Wednesday, August 08, 2012 5:48 AM
To: Lee & Peggy Wenk; contact <@t> histocare.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] tissue highlighting for visibility
ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop
Kate Mendell
Histopathology/Lab Manager
HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA 01907
TEL: 781.595.0151
FAX: 781.592.6780
kmendell <@t> goldbergmd.net
www.cosmesticdermcenter.com
PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk <@t> sbcglobal.net]
Sent: Wednesday, August 08, 2012 5:33 AM
To: contact <@t> histocare.com; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] tissue highlighting for visibility
Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
The opinions expressed are mine, and do not reflect those of Beaumont Hospital.
-----Original Message-----
From: contact <@t> histocare.com
Sent: Tuesday, August 07, 2012 6:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue highlighting for visibility
Hello all,
Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort.
I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do?
www.HistoCare.com
Histology Staffing
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Wed, 8 Aug 2012 07:25:22 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Tissue Processor
To: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1344435922.89103.YahooMailNeo <@t> web121405.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Sakura
Ren? J.
________________________________
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, August 8, 2012 8:11 AM
Subject: [Histonet] Tissue Processor
I am going to need to purchase a new tissue processor mine keeps breaking down.? What tissue processor would you buy and why?? I would greatly appreciate the help.
Cheers,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you."
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------------------------------
Message: 18
Date: Wed, 8 Aug 2012 07:32:39 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] tissue highlighting for visibility
To: "contact <@t> histocare.com" <contact <@t> histocare.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1344436359.627.YahooMailNeo <@t> web121402.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I always used few drops of alcoholic eosin in the 70%EthOL, just enough to make the solution a "pale pink". That amount is enough to give a faint "pink hue" to the tissue to ease its localization.
This does not interfere with any stain done after wards.
Ren? J.
________________________________
From: "contact <@t> histocare.com" <contact <@t> histocare.com>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, August 7, 2012 6:10 PM
Subject: [Histonet] tissue highlighting for visibility
Hello all,
Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it,
a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort.
I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ?What do some of you guys do?
http://www.histocare.com/
Histology Staffing
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Wed, 8 Aug 2012 09:29:29 -0500
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] decalcification of premolar teeth (dog)
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388D4F <@t> UTHCMS1.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Alice
I would recommend using sodium formate/formic acid mixture for demineralization as this is more gentle than most agents.
I would not use hydrochloric acid unless you are shipwrecked on a desert island and that is the only chemical available to you.
I am assuming that EDTA demineralization is not an option for you?
Barry
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alice Fraser [toxpathnz <@t> gmail.com]
Sent: Tuesday, August 07, 2012 8:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decalcification of premolar teeth (dog)
Dear Members
I would be really interested to hear your advice on the currently preferred
procedure for decalcification of premolar teeth from dogs. Do laboratories
find the HCl/water solution or the formic acid solution or another solution
to be optimal for decal without obliterating the tissues to be evaluated? A
procedure/method would be hugely appreciated if poss.
Many thanks.
Alice
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------------------------------
Message: 20
Date: Wed, 8 Aug 2012 07:48:32 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] help ! paraffin section
To: Megha Kumar <meghak <@t> g.clemson.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1344437312.51180.YahooMailNeo <@t> web121405.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
What you describe is a typical example of poor paraffin infiltration = the paraffin has not infiltrated the tissue and when you prepare the final block it will consist of 2 different components; the tissue and the paraffin. That is why you end with a "good paraffin section" without the tissue.
Poor paraffin infiltration is always caused by an improper sequence while tissue processing. Either the fixation is incomplete
OR the dehydration is incomplete and there is water in the tissue when you go to the "clearing" stage
OR the clearing stage is incomplete and the tissue still has alcohol (immiscible with paraffin) when the tissue goes to the paraffin
OR the paraffin infiltration is too short.
The problem resides in your processing protocol and there is nothing you can do about that?at the end.
Try to check your processing protocol to eliminate the problem.
If this is happening "all of the sudden" while you used to have good results previously, then you either have changed reagents or the reagents?are not in a good condition.
Ren? J.?
________________________________
From: Megha Kumar <meghak <@t> g.clemson.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, August 7, 2012 11:45 PM
Subject: [Histonet] help ! paraffin section
Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding. However, when i section, the tissue is torn although the rest of
the paraffin looks perfect. Please suggest why this is happening. Also,
sometimes the skin sections fall off the slides when I perform in situ
hybridization. Any ideas how to prevent this?
Please help! i am a beginner in histology and dont' know what to do!
regards
Megha
*
*
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End of Histonet Digest, Vol 105, Issue 9
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