[Histonet] Ki67 antibody to stain Mouse tissue

[Jan Shivers]

Eva, et al,

Yes, the LabVision/ThermoSci rabbit polyclonal does stain mouse tissue.
 I've done validation trials on various species with the antibody and mouse
was one of many that did react.

Jan Shivers
UMN Vet Diag Lab

On Wed, Aug 8, 2012 at 10:51 AM, Eva Permaul <eca9 <@t> georgetown.edu> wrote:

> I just found a nice publication with the Rm-9106 on mouse intestine. Have
> provided it to my manager. Thank you everyone.
> Eva
>
> On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul <eca9 <@t> georgetown.edu> wrote:
>
> > Just to clarify. Are you using it on mouse tissues? I am only asking
> > because the Thermo Scientific/Labvision/Neomarkers data sheet says it has
> > only been verified on Human tissues.
> > Secondly had anyone used it on mouse gut. Does it stain in the bottom of
> > the crypts or at the top? I am asking because I have also tried a
> different
> > antibody from Leica but got staining at the top of the crypts not at the
> > bottom.
> >
> > Thank you to everyone for all of your valuable suggestions and advice,
> > Eva Permaul
> > Georgetown University
> >
> >
> > On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) <
> > joost.bruijntjes <@t> tno.triskelion.nl> wrote:
> >
> >> Hi Eva
> >>
> >> I've used an antibody from Labvision /Neomarkers. It is a rabbit
> >> monoclonal; catalog number: RM-9106.
> >>
> >> Joost
> >>
> >> -----Oorspronkelijk bericht-----
> >> Van: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> >> histonet-bounces <@t> lists.utsouthwestern.edu] Namens Eva Permaul
> >> Verzonden: maandag 6 augustus 2012 20:44
> >> Aan: histonet
> >> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue
> >>
> >> Hello,
> >> I am looking for a Ki67 to stain mouse tissues. We have been using Dako
> >> M2749 but just found out it has been discontinued. Could anyone suggest
> a
> >> good alternative?
> >> Thank you all for any help,
> >> Eva Permaul
> >> Georgetown University
> >> _______________________________________________
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> >>
> >
> >
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