[Histonet] RE: counterstain for Alcian Blue (ph2.5)

[Tony Henwood (SCHN)]

Try a weak Harris's Haematoxylin stain (half the normal stain time in your H&E).
The two blues are easy to distinguish.

Harris's is preferred to Mayer's since Harris's seems to have a dark purple hue compared to the sky blue of Mayer's.

You could even use a 1% neutral red in 1% acetic acid or even just a light eosin counterstain.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Thursday, 2 August 2012 2:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] counterstain for Alcian Blue (ph2.5)

Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution

 

Diana

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