[Histonet] RE: counterstain for Alcian Blue (ph2.5)

[Smith, Allen]

The time and trouble of making up a solution of aluminum mordanted nuclear fast red repays you well.  The staining solution contains to work well for an entire year.
If you must have something else, brazalum, made by substituting brazilin for hematoxylin in Mayer's hemalum, works very well.  The solution keeps its staining qualities for 2 or 3 months.  Anatech sells brazalum ready-made as "Brazilliant."  
Although Orth's lithium carmine gives beautiful results, I don't recommend it: it's a pain to make and keeps its staining power for only 2 weeks.
Allen A. Smith
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, FL

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, August 01, 2012 12:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] counterstain for Alcian Blue (ph2.5)

Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution

 

Diana

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