[Histonet] Re: Histonet Digest, Vol 101, Issue 23

[Madeleine Huey]

Clare,

I have NO background when with polyclonal Rabbit anti-PAX 8 from Cell
Marque (RTU) & monoclonal Mouse anti-PAX 8 from Biocare (RTU) on Leica
Bond nor Dako Plus (never Ventana - need prep kit).

Rabbit-PAX 8 is more sensitive, but the mouse-PAX 8 is more specific,
but both work well.

Maybe we can help you if you give us a more detail protocol.

Best,
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleinehuey <@t> elcaminohospital.org





> Date: Wed, 18 Apr 2012 10:04:35 -0400
> From: Clare Thornton <CThornton <@t> dahlchase.com>
> Subject: [Histonet] PAX-8 antibody
> To: "'histonet <@t> lists.utsouthwestern.edu'"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <C9D78FFC9D668B4CBEA4405F846975040109B08CAC0F <@t> iris.dahlchase.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Looking for help with a protocol for this antibody using Ventana platform, either Ultra or Benchmark, Ultraview detection and Biocare's predilute antibody.  Would like protocol for any other vendor's antibody as well.  We are having issues with light staining and too much background.
>
> thank you!
>
> Clare J. Thornton, HTL(ASCP), QIHC
> Assistant Histology Supervisor
> Dahl-Chase Diagnostic Services
> 417 State Street, Suite 540
> Bangor, ME 04401
> cthornton <@t> dahlchase.com
>
>
>
> ------------------------------