[Histonet] RE: Wrinkles in whole bone mouse tibias

[Meghana Bapardekar]

Yes it should work, placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes as we experienced with similar samples. Hope this helps! 

Meghana V. Bapardekar, Ph. D.,
Dept. of Histopathology,
Cleveland Biolabs, 73 High Street,
Buffalo, NY - 14203.
Phone: 716-849-6810, ext.: 357
Fax: 716-849-6817

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Machin, Leisha E
Sent: Friday, April 13, 2012 2:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias

Dear Nancy,

I had your mouse tibia problem last year; the marrow consistently folded up accordion-style.  I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes.

Hope this help - best of luck!

Leisha Machin


 


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