[Histonet] Re: Wrinkles in whole bone mouse tibias
Machin, Leisha E
Leisha_Machin <@t> URMC.Rochester.edu
Fri Apr 13 13:24:46 CDT 2012
Dear Nancy,
I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes.
Hope this help - best of luck!
Leisha Machin
"The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas
________________________________________
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Subject: Histonet Digest, Vol 101, Issue 18
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Today's Topics:
1. ACMS perspective on Aetna's new requirments. (Nicole Tatum)
2. Prepared STD slides? (Jon Krupp)
3. HistoTALK at the Region III Meeting (David Kemler)
4. Competency assesment forms for Pathologists (Sheila Adey)
5. Re: Prepared STD slides? (Kim Donadio)
6. Re: Prepared STD slides? (Lee & Peggy Wenk)
7. "know error system" - prostate biopsies (Richard Cartun)
8. RE: "know error system" - prostate biopsies (Lester Raff MD)
9. Formalin asfety question (O'Donnell, Bill)
10. Wrinkles in whole bone mouse tibias (nancy lowen)
11. RE: "know error system" - prostate biopsies (Heath, Nancy L.)
12. RE: Formalin asfety question (O'Donnell, Bill)
13. RE: New Reagent Lot Verification (Settembre, Dana)
14. Re: Wrinkles in whole bone mouse tibias (Louise Renton)
----------------------------------------------------------------------
Message: 1
Date: Thu, 12 Apr 2012 13:32:30 -0400 (EDT)
From: "Nicole Tatum" <nicole <@t> dlcjax.com>
Subject: [Histonet] ACMS perspective on Aetna's new requirments.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2628.208.62.167.196.1334251950.squirrel <@t> webmail.realpages.com>
Content-Type: text/plain;charset=iso-8859-1
This is a letter From the American College of Mohs Surgery in reguards to
Aetna letter for CAP requiremnet.
April 5, 2012
I read your letter of March 23, 2012, outlining Aetna?s new requirement
for CLIA and CAP certification for certain pathology services with dismay.
I am very concerned these requirements could lead to lower cure rates,
increased tissue loss and scarring, and even unnecessary deaths in your
patients with problem skin cancer with Aetna insurance.
CAP certification will be almost impossible to achieve for small Mohs
Micrographic surgery frozen section laboratories. These labs can only be
CAP certified if directed by an anatomic pathologist or
dermatopathologist. Mohs surgeons, who have extensive additional training
in pathology, and training to run their laboratories, do not fit into
either niche. Mohs surgeons deal with the most difficult and recurrent
skin cancers.
Please find attached a copy of the CPT coding assistant from 2008
describing Mohs surgery, and detailing what services are included in it.
With your new restrictions, immunohistochemistry (CPT code 88342) cannot be
billed with Mohs surgery for malignant melanoma and spindle cell tumors.
This will restrict the use of Mart 1 immuno stains and other special
cytokeratin stains. This will result in lower cure rates and an increased
number of deaths. These stains on additional frozen sections are not part
of Mohs surgery, and are appropriately billed for separately.
In addition, Mohs surgeons will not be able to bill for the occasional
special stains on frozen sections (CPT code 8814) such as oil red O on a
sebaceous carcinoma, or toluidine blue (in addition to the hematoxylin and
Eosin stained frozen sections) to clarify an area of inflammation.
Performing these special stains on ambiguous frozen sections often saves
the patient additional surgery and tissue loss, and saves
Aetna money, because another stage of Mohs surgery is avoided. These
additional stains are not part of the Mohs surgery and are appropriately
billed for separately.
Denial of these CPT codes may result in more tissue being removed
unnecessarily, lower cure rates, higher recurrence rates, and potentially
deaths.
Quality control of the frozen section laboratory is crucial, and mandatory
for CAP lab approval. This quality control involves processing of
?confirming? formalin sections off the frozen blocks of tissue is commonly
performed in Mohs surgery laboratories for quality control and
confirmation. This will no longer be a billable service (CPT
code 88305) per your letter.
This directly contradicts CAP own recommendations for the follow
up processing of frozen sections (see attached). These confirming formalin
sections are not part of Mohs surgery and are separately billable. Your
decision not to cover code CPT 88305 makes it impossible, or at a minimum,
fiscally prohibitive, to maintain quality control and to even
consider complying with the new CAP accreditation you are demanding in the
same letter.
>From the perspective of the American College of Mohs surgery, our patients
could continue to receive the best quality of care, and the overall cost
to Aetna may actually be lower (by avoiding additional stages of Mohs
surgery), if CPT Codes 88314, 88305, and 88342 were exempted for
providers who also bill the Mohs surgery CPT codes 17311 or 17313.
Sincerely
Brett Coldiron, M.D., F.A.C.P.
President American College of Mohs Surgery
------------------------------
Message: 2
Date: Thu, 12 Apr 2012 12:48:34 -0700
From: Jon Krupp <jkrupp <@t> deltacollege.edu>
Subject: [Histonet] Prepared STD slides?
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <C48EDCE7-468B-4D7C-8D84-5A2A12AC892A <@t> deltacollege.edu>
Content-Type: text/plain; charset=us-ascii
Hi
I not a histo tech, nor do I play one on TV, so I might not be up to speed on this.
A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class.
Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish.
Thanks
Jon
Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA 95207
209-954-5284
jkrupp <@t> deltacollege.edu
Find us on Facebook @
Electron Microscopy at SJ Delta College
------------------------------
Message: 3
Date: Thu, 12 Apr 2012 13:32:45 -0700 (PDT)
From: David Kemler <histotalk <@t> yahoo.com>
Subject: [Histonet] HistoTALK at the Region III Meeting
To: Fellow HistoNetters <Histonet <@t> Lists.UTSouthwestern.edu>
Message-ID:
<1334262765.35947.YahooMailNeo <@t> web120602.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi HistoNetters - Tomorrow the HistoTALK "mobile" studio will be arriving late in the day?for the Region III Meeting at?Callaway Gardens, in Pine Mountain, GA,?We'll be there?to interview (it is not LIVE)?everyone and anyone daring to go before the HistoTALK microphone to be a guest on a later broadcast show. Even if you don't care to be a guest on the show, plan on stopping by on Saturday to say hi.
?
There are plenty of open interview?times available on Saturday, so don't be shy!
?
See you at the Region III Meeting which is hosted by my Georgia Society for Histotechnology friends.
?
Yours,
David?
------------------------------
Message: 4
Date: Thu, 12 Apr 2012 16:50:46 -0400
From: Sheila Adey <sadey <@t> hotmail.ca>
Subject: [Histonet] Competency assesment forms for Pathologists
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY154-W39BA84CF7EF42548043F05C63A0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hello netters:Does anyone have an example of a competency assessment form designed for pathologists?Thanks in advance:)Sheila
------------------------------
Message: 5
Date: Thu, 12 Apr 2012 21:03:30 -0400
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] Prepared STD slides?
To: Jon Krupp <jkrupp <@t> deltacollege.edu>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6E088F6E-6D78-43DD-A3A3-D9F2772C80E3 <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
Contact your local college
Might help
Sent from my iPhone
On Apr 12, 2012, at 3:48 PM, Jon Krupp <jkrupp <@t> deltacollege.edu> wrote:
> Hi
>
> I not a histo tech, nor do I play one on TV, so I might not be up to speed on this.
>
> A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class.
>
> Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish.
>
> Thanks
>
> Jon
>
> Jonathan Krupp
> Delta College
> 5151 Pacific Ave.
> Box 212
> Stockton, CA 95207
> 209-954-5284
> jkrupp <@t> deltacollege.edu
>
> Find us on Facebook @
> Electron Microscopy at SJ Delta College
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Fri, 13 Apr 2012 05:03:33 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Prepared STD slides?
To: "Kim Donadio" <one_angel_secret <@t> yahoo.com>, "Jon Krupp"
<jkrupp <@t> deltacollege.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <2DBC101318AF4479B45C1457B9AF4432 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
A Google search of "prepared histology slides" found a company called
Carolina
http://www.carolina.com/
Click Life Sciences
Click Microscopic slides
Don't know if they have exactly what you want, but it's a start.
Don't know anything about the company. Maybe someone on Histonet does.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
(Opinions expressed are mine, and do not reflect my place of employment.)
-----Original Message-----
From: Kim Donadio
Sent: Thursday, April 12, 2012 9:03 PM
To: Jon Krupp
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Prepared STD slides?
Contact your local college
Might help
Sent from my iPhone
On Apr 12, 2012, at 3:48 PM, Jon Krupp <jkrupp <@t> deltacollege.edu> wrote:
> Hi
>
> I not a histo tech, nor do I play one on TV, so I might not be up to speed
> on this.
>
> A microbiology instructor asked me to see if there is a source of prepared
> slides of STD's that he could use in class.
>
> Maybe you know some place we could check? I have done some WWW searching,
> but have had a hard time finding prepared slides students could use with a
> microscope in class. Most everything seems to have gone digital and
> PowerPoint-ish.
>
> Thanks
>
> Jon
>
> Jonathan Krupp
> Delta College
> 5151 Pacific Ave.
> Box 212
> Stockton, CA 95207
> 209-954-5284
> jkrupp <@t> deltacollege.edu
>
> Find us on Facebook @
> Electron Microscopy at SJ Delta College
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 7
Date: Fri, 13 Apr 2012 09:20:32 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] "know error system" - prostate biopsies
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4F87EFE0.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
------------------------------
Message: 8
Date: Fri, 13 Apr 2012 08:24:43 -0500
From: "Lester Raff MD" <LRaff <@t> uropartners.com>
Subject: RE: [Histonet] "know error system" - prostate biopsies
To: "Richard Cartun" <Rcartun <@t> harthosp.org>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE0368FE5AA6684DB5990BBEED742C53010BDD7B <@t> UPHQMSX01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
We do not!
Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.492.0203
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, April 13, 2012 8:21 AM
To: Histonet
Subject: [Histonet] "know error system" - prostate biopsies
I am curious how many of you working in hospital or non-hospital
Anatomic Pathology labs are using the "know error system" for prostate
biopsy/patient DNA confirmation? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Fri, 13 Apr 2012 07:26:46 -0600
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
Subject: [Histonet] Formalin asfety question
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4940DF6D1C5FDF48931B6966AAEF939553201E <@t> chimsx08.CHI.catholichealth.net>
Content-Type: text/plain; charset="ISO-8859-1"
Is formalin an RCRA regulated waste? I am having some difficulty getting
this answered and figured someone who is much brighter than myself out
there may well know. If anyone has a reference, that would be a huge
help
William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
SERENITY is not freedom from the storm, but peace amid the storm.
Cultivate it in PRAYER!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, April 13, 2012 8:21 AM
To: Histonet
Subject: [Histonet] "know error system" - prostate biopsies
I am curious how many of you working in hospital or non-hospital
Anatomic Pathology labs are using the "know error system" for prostate
biopsy/patient DNA confirmation? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
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------------------------------
Message: 10
Date: Fri, 13 Apr 2012 06:30:49 -0700 (PDT)
From: nancy lowen <claycal44 <@t> yahoo.com>
Subject: [Histonet] Wrinkles in whole bone mouse tibias
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1334323849.98400.YahooMailNeo <@t> web164503.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello to all,
I am having on ongoing problem with wrinkles in the midshaft marrow area of sections of whole mouse tibias.? Leaving them in the waterbath longer does not help--water bath temp. is 48 to 49 degrees.? I have tried several waxes to help, and am currently using McCormick Paraplast Plus.
Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes for 1hour, 1hour,1hour, and 3hours for the last.
Specimens seem to cut fine, and the head of the bone is good, but the wrinkles are in the middle to lower part of the diaphysis.
Is is possible that the polymers in the wax could be the problem?
I see it mostly in the thinner, densely maarrow packed bones.
If anyone has any ideas, could you please pass it along, as I am getting gray hairs from trying to figure it out.
Also, I do not fix the bones myself, but most of the bones I get have been fixed for 24 hours in 10% Formalin.
Thanks in advance.
Nancy.lowen <@t> Va.gov
------------------------------
Message: 11
Date: Fri, 13 Apr 2012 09:41:01 -0400
From: "Heath, Nancy L." <NHeath <@t> Lifespan.org>
Subject: RE: [Histonet] "know error system" - prostate biopsies
To: "Richard Cartun" <Rcartun <@t> harthosp.org>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<130E8991F210424096EFC6F42EA33B2408D75B99 <@t> LSCOEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="us-ascii"
When I worked per-diem for Plus Diagnostics here in Warwick RI we used
it. It was a pain in the butt!!
Nancy Heath, HT (ASCP)
Neuropathology Technician
Pathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology
Rhode Island Hospital
APC Blding, Flr 12, Rm 211
593 Eddy Street
Providence, RI 02903
lab: 401-444-3246
fax: 401-444-8514
nheath <@t> lifespan.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, April 13, 2012 9:21 AM
To: Histonet
Subject: [Histonet] "know error system" - prostate biopsies
I am curious how many of you working in hospital or non-hospital
Anatomic Pathology labs are using the "know error system" for prostate
biopsy/patient DNA confirmation? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Fri, 13 Apr 2012 08:12:11 -0600
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
Subject: RE: [Histonet] Formalin asfety question
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4940DF6D1C5FDF48931B6966AAEF9395532039 <@t> chimsx08.CHI.catholichealth.net>
Content-Type: text/plain; charset="ISO-8859-1"
I withdraw the inquiry. Thanks
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Friday, April 13, 2012 8:27 AM
To: Histonet
Subject: [Histonet] Formalin asfety question
Is formalin an RCRA regulated waste? I am having some difficulty getting
this answered and figured someone who is much brighter than myself out
there may well know. If anyone has a reference, that would be a huge
help
William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good
Samaritan Hospital 10 East 31st Street Kearney, NE 68847
SERENITY is not freedom from the storm, but peace amid the storm.
Cultivate it in PRAYER!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, April 13, 2012 8:21 AM
To: Histonet
Subject: [Histonet] "know error system" - prostate biopsies
I am curious how many of you working in hospital or non-hospital
Anatomic Pathology labs are using the "know error system" for prostate
biopsy/patient DNA confirmation? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This electronic mail and any attached documents are intended solely for
the named addressee(s) and contain confidential information. If you are
not an addressee, or responsible for delivering this email to an
addressee, you have received this email in error and are notified that
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received this email in error, immediately reply to the sender and delete
the message completely from your computer system.
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This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
------------------------------
Message: 13
Date: Fri, 13 Apr 2012 11:02:31 -0400
From: "Settembre, Dana" <settembr <@t> umdnj.edu>
Subject: RE: [Histonet] New Reagent Lot Verification
To: "'Sebree Linda A'" <lsebree <@t> uwhealth.org>, "Rathborne, Toni"
<trathborne <@t> somerset-healthcare.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B64B947688FB794A8C191D19F22927C805738CA79D <@t> UMDEXMBX02.core.umdnj.edu>
Content-Type: text/plain; charset="us-ascii"
We are saving slides from inspection to inspection, about 2 years.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Thursday, April 12, 2012 11:26 AM
To: Rathborne, Toni; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] New Reagent Lot Verification
We're saving both for at least 2 years.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Thursday, April 12, 2012 9:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Reagent Lot Verification
ANP.22760 refers to new lot verification for antibodies and detection.
The Evidence of Compliance says that there should be "Records of
verification of new reagent lots". What is everyone's interpretation of
"records"? Are you saving slides in addition to paper documentation?
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------------------------------
Message: 14
Date: Fri, 13 Apr 2012 18:34:42 +0200
From: Louise Renton <louise.renton <@t> gmail.com>
Subject: Re: [Histonet] Wrinkles in whole bone mouse tibias
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CABL-0cDSF+PucERGMzChOkSeZ=XvK3=NdDHER0ogq0OOVn0Hnw <@t> mail.gmail.com>
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Dear nancy
what is the melting temp of the wax? perhaps your waterbath is too cool.
Ideally you have have the temp so that the section expands but does not
disintegrate.
You could also try to pick up the wrinkly section on a slide, and then sort
of "dab" it on a hotplate to "steam" out the wrinkles before you set the
slide to drain.
regards
On Fri, Apr 13, 2012 at 3:30 PM, nancy lowen <claycal44 <@t> yahoo.com> wrote:
> Hello to all,
> I am having on ongoing problem with wrinkles in the midshaft marrow area
> of sections of whole mouse tibias. Leaving them in the waterbath longer
> does not help--water bath temp. is 48 to 49 degrees. I have tried several
> waxes to help, and am currently using McCormick Paraplast Plus.
> Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100%
> alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes
> for 1hour, 1hour,1hour, and 3hours for the last.
> Specimens seem to cut fine, and the head of the bone is good, but the
> wrinkles are in the middle to lower part of the diaphysis.
> Is is possible that the polymers in the wax could be the problem?
> I see it mostly in the thinner, densely maarrow packed bones.
> If anyone has any ideas, could you please pass it along, as I am getting
> gray hairs from trying to figure it out.
> Also, I do not fix the bones myself, but most of the bones I get have been
> fixed for 24 hours in 10% Formalin.
> Thanks in advance.
> Nancy.lowen <@t> Va.gov
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--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
Question: Are rhinos overweight unicorns?
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