[Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?

[Francis OBrien]

I would be using the freezing stage with dry ice.

On Fri, Sep 23, 2011 at 3:33 PM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Unless you have means to maintain the frozen tissue frozen, you will not be
> able to do it because the tissue will thaw and sectioning will be
> impossible.
> René J.
>
>
> --- On *Fri, 9/23/11, Francis OBrien <francisobrien2007 <@t> gmail.com>* wrote:
>
>
> From: Francis OBrien <francisobrien2007 <@t> gmail.com>
> Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, September 23, 2011, 12:03 PM
>
> Hello,
>
> I am a newbie to this type of work. We have flash frozen tissue in OCT
> (Optimal Cutting Temperature) compound for some unique experiments
> that we need to carry out. We want to use a AO-860 sliding microtome
> to cut large slabs of OCT embedded tissue. Does anyone have any advice
> on how this would work reproducibly and reliably on the freezing
> stage.
>
> Best,
>
> Francis
>
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>


-- 
Regards,

Francis

Research graduate,
New York School of Medicine
New York