[Histonet] Uni-trieve, antigen retrieval for immunofluorescence

[sdysart <@t> mirnarx.com]

I actually attended a class they put on a few months ago where they used
Unitrieve.  It works really well.  It doesn't seem to produce any kind
of background and yes, it is used at much lower temps.  However the
retrieval process takes a little longer, but...you can put it in an even
lower temp. water bath overnight, come in to work in the morning, and
your slides are reading to go.
Contact Zara over there and she might be able to hook you up with a
sample.
Good Luck!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Marion
Sent: Tuesday, September 20, 2011 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

Hello all,

Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
to
be a universal antigen retrieval solution that can be used at lower
temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):

http://innvx.com/unitrievepage.html

The company claims that the reagent is a universal retrieval solution
for
all antibodies and tissues (which is silly of course - how could they
know?). Does anyone have any experience with the product?

I am interested because I see that increased heat during antigen
retrieval
causes greater tissue autofluorescence during immunofluorescence
stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
plate using sodium citrate buffer. Does anyone else either Uni-trieve or
a
different reagent/protocol for immunofluorescence stainings?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago


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