[Histonet] Uni-trieve, antigen retrieval for immunofluorescence

[Andrea Marion]

Hi Sarah,

Thanks for the info! Have you used Unitrieve for immunofluorescence? I am
wondering whether I will see a reduction in the autofluorescence caused by
heating? I will request a sample to try and report back to all.

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago

On Tue, September 20, 2011 12:49 pm, sdysart <@t> mirnarx.com wrote:
> I actually attended a class they put on a few months ago where they used
> Unitrieve.  It works really well.  It doesn't seem to produce any kind
> of background and yes, it is used at much lower temps.  However the
> retrieval process takes a little longer, but...you can put it in an even
> lower temp. water bath overnight, come in to work in the morning, and
> your slides are reading to go.
> Contact Zara over there and she might be able to hook you up with a
> sample.
> Good Luck!!
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
> Marion
> Sent: Tuesday, September 20, 2011 11:59 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
>
> Hello all,
>
> Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
> to
> be a universal antigen retrieval solution that can be used at lower
> temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
> antigens):
>
> http://innvx.com/unitrievepage.html
>
> The company claims that the reagent is a universal retrieval solution
> for
> all antibodies and tissues (which is silly of course - how could they
> know?). Does anyone have any experience with the product?
>
> I am interested because I see that increased heat during antigen
> retrieval
> causes greater tissue autofluorescence during immunofluorescence
> stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
> plate using sodium citrate buffer. Does anyone else either Uni-trieve or
> a
> different reagent/protocol for immunofluorescence stainings?
>
> Thanks,
>
> Andrea
>
> Andrea Marion
> Graduate Student
> University of Illinois at Chicago
>
>
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