[Histonet] H&E

[Jennifer Campbell]

We use ProPar on our stainer. We rotate our 4 100% alcohols that are after
eosin after every 3rd rack and we have stopped the leaching of eosin in our
last ProPars.

Not a big fan of exposing my staff to xylene unless I absolutely have to.

Jen Campbell

On Thu, Sep 15, 2011 at 9:44 AM, <sdysart <@t> mirnarx.com> wrote:

> This happened to me several months ago.  Our lab always has high humidity.
>  If you are going to continue to use clear rite with humidity in the air, I
> would change it everyday.  Also, change out your 100% because it's
> inevitably getting water in it too.  I just switched my whole lab back to
> xylene...at least you can see the water in it =)
> Good Luck
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anuradha
> shrivastava
> Sent: Wednesday, September 14, 2011 9:35 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] H&E
>
> Hello everybody,
> Can anyone help me, In Leica autostainer we have after eosin 3 100% series,
> clear-rite/100% and then 3 clear rites. I observed that during humid days
> eosin was leaching . I replaced 2 clear rite after 50 :50
> Abs/cler. with xylene. The slides were fine after wards. My question is ,
> is it ok to keep the last clear rite or I should change that too to xylene.
> We had never used xylene before in the stainer. The coverslipper’s bath is
> filled with the clear rite.( xylene substitute), Please give your expert
> advice. Thanks .
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-- 
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137