[Histonet] H&E

[sdysart <@t> mirnarx.com]

This happened to me several months ago.  Our lab always has high humidity.  If you are going to continue to use clear rite with humidity in the air, I would change it everyday.  Also, change out your 100% because it's inevitably getting water in it too.  I just switched my whole lab back to xylene...at least you can see the water in it =)
Good Luck

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anuradha shrivastava
Sent: Wednesday, September 14, 2011 9:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E

Hello everybody,
Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50
Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper’s bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks .
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