[Histonet] TEM using "difficult" tissue

[Jan Shivers]

Daphne,

We do EM work on formalin-fixed, paraffin-embedded tissue occasionally.  I 
will send you a brief protocol under separate cover.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Daphne Gill" <dagill <@t> upei.ca>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 13, 2011 11:17 AM
Subject: [Histonet] TEM using "difficult" tissue


> Hi
> I'd like to try some TEM work on rat brain hippocampus. However, the
> tissue is less than ideal. Does anyone have any information/insight on
> using old (harvested ~2 years ago) tissue that has been subjected to 20
> minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
> 48 hours, and then frozen?
> Thanks very much for any help you can give me.
>
> Daphne
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