[Histonet] TEM using "difficult" tissue

[Geoff McAuliffe]

I suspect it will be somewhere between rather ugly to totally worthless, 
more likely the latter.
The only was to know is to find out for yourself. Thaw, rinse multiple 
times in buffer, osmicate in buffer, rinse, dehydrate, clear and embed 
in your favorite epoxy.
Good luck.

Geoff

On 9/13/2011 12:17 PM, Daphne Gill wrote:
> Hi
> I'd like to try some TEM work on rat brain hippocampus. However, the
> tissue is less than ideal. Does anyone have any information/insight on
> using old (harvested ~2 years ago) tissue that has been subjected to 20
> minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
> 48 hours, and then frozen?
> Thanks very much for any help you can give me.
>
> Daphne
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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