[Histonet] immunohistochemistry: manual staining

[Thomas, Nancy]

We use the Sequenza immunostaining center available through Thermo.  The slides are inserted upright, with an attached coverplate.  This keeps the slides from drying out and also reduces the amount of reagents needed.  

Nancy Thomas 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim Van den Broeck
Sent: Tuesday, September 13, 2011 7:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining

Dear Colleagues,

We do a lot of immunohistochemical stainings (research, no diagnostics), but unfortunately, we cannot afford an automated stainer. At the moment, we do everything manually (washing, incubating, ....) but I would like to make things run more smoothly. So my question is, is there any device or systems that can be helpful during manual IHC stainings ? 

Thanks in advance for your comments / suggestions.

--
Met vriendelijke groeten,


Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
wim.vandenbroeck <@t> UGent.be - tel.: +32 9 264 77 16 - fax: +32 9 264 77 90


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