[Histonet] How to save dried antibody?

[Goins, Tresa]

If the antibody has precipitated, it may be impossible to restore activity.  The glycerol is present to keep the suspension in liquid form - and it sounds like you no longer have a liquid.  Try adding an appropriate suspension buffer and keep your fingers crossed.  If you do not have previous experience with this antibody and know what to expect regarding performance, I would discard it and re-order.

Tresa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sarah Tabatabaei
Sent: Friday, October 28, 2011 9:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] How to save dried antibody?



Dear All,

We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff 
and looks like it's being crystallized. I cannot pipet any of it. It 
has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the 
antibody doesn't seem to be mixing with it.

Does anyone know how to save this antibody? How can I bring it back to its liquid state? 

Thank you for your time

 
Sarah Sadat, DDS


MSc Student, Dental Sciences
McGill University
Montreal QC.

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