[Histonet] Suggestions please...

[Lee & Peggy Wenk]

Is there a slaughter house nearby? Call them, and have some documentation 
that you are from a university - such as a memo on a letterhead.

Is there animal research at your university? Can they spare a rat?

Try to do this right before class, so there is less autolysis. Put tissue in 
a plastic bag, and store in refrig for a few hours, until ready.

Peggy Wenk, HTL(ASCP)SLS

-----Original Message----- 
From: Komal Gada
Sent: Saturday, October 22, 2011 9:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Suggestions please...

Dear Histonetters,

I'm currently teaching Histology at a University, and I was hoping for some
suggestions on how to teach students to use a cryostat. I have several
questions:

1) Since we do not have access to actual specimens, what would any of you
recommend could be used as a viable option?

So far, I'm thinking either hot dogs or chicken breasts, but please feel
free to suggest what you think and why so that I can understand the logic.

2) Which post-fixative should I use and how long?

3) Are there any suggestions for the H&E staining procedure?

Thanks!
Komal
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