AW: [Histonet] Suggestions please...

[Gudrun Lang]

I suggest fresh pig-kidney, if you can get it at the butcher.

Kidney is relatively easy to cut and you can prepare small quaders of 2-3 mm
thickness, that are frozen fast enough directly in the cryocut.
After cutting you can wrap it in foil and store it in the fridge (-20°C) for
the next students.

For teaching I think air drying is good enough before HE-staining.
Alternatively you can take 37-40% formaldehyde for a few seconds and rinsing
in tapwater afterwards.

Then 1 min Harris hematoxylin
Rinse in acidified water (dest. water + 1 drop acetic acid)
Rinse in tapwater until blue enough
Rinse in 96% ethanol
15-30 seconds in 2% eosin in 96% ethanol
Rinse in 96% ethanol
2 x rinse in absolute ethanol
1 x rinse in xylol or substitute
Coverslipping with resin-media

Hope that helps
Gudrun


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Komal Gada
Gesendet: Samstag, 22. Oktober 2011 15:46
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Suggestions please...

Dear Histonetters,

I'm currently teaching Histology at a University, and I was hoping for some
suggestions on how to teach students to use a cryostat. I have several
questions:

1) Since we do not have access to actual specimens, what would any of you
recommend could be used as a viable option?

So far, I'm thinking either hot dogs or chicken breasts, but please feel
free to suggest what you think and why so that I can understand the logic.

2) Which post-fixative should I use and how long?

3) Are there any suggestions for the H&E staining procedure?

Thanks!
Komal
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