[Histonet] Suggestions please...

[Sheila Adey]

You could buy some beef liver from the grocery store. Works great. 
 

> From: lpwenk <@t> sbcglobal.net
> To: kjgada <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Date: Sun, 23 Oct 2011 08:18:12 -0400
> Subject: Re: [Histonet] Suggestions please...
> CC: 
> 
> Is there a slaughter house nearby? Call them, and have some documentation 
> that you are from a university - such as a memo on a letterhead.
> 
> Is there animal research at your university? Can they spare a rat?
> 
> Try to do this right before class, so there is less autolysis. Put tissue in 
> a plastic bag, and store in refrig for a few hours, until ready.
> 
> Peggy Wenk, HTL(ASCP)SLS
> 
> -----Original Message----- 
> From: Komal Gada
> Sent: Saturday, October 22, 2011 9:46 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Suggestions please...
> 
> Dear Histonetters,
> 
> I'm currently teaching Histology at a University, and I was hoping for some
> suggestions on how to teach students to use a cryostat. I have several
> questions:
> 
> 1) Since we do not have access to actual specimens, what would any of you
> recommend could be used as a viable option?
> 
> So far, I'm thinking either hot dogs or chicken breasts, but please feel
> free to suggest what you think and why so that I can understand the logic.
> 
> 2) Which post-fixative should I use and how long?
> 
> 3) Are there any suggestions for the H&E staining procedure?
> 
> Thanks!
> Komal
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