[Histonet] RE: Substitute Xylene for Bone tissue

[Marcum, Pamela A]

We used a xylene substitute (non-aliphatic Hydrocarbon not d-limonene) in with large animal bone and it was a great help.  We always used the first clearing as a xylene for the defatting quality and assistance it gave to the overall specimen.  It was explained to me by someone at the time that the xylene does the defatting and as we all know tends to harden bone to some extend while the substitute will (to an extent) allow some softening of the bone.  This does not mean it gets too soft to use with MMA only just a better section in the end.

Pam Marcum

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jack Ratliff
Sent: Thursday, October 13, 2011 11:51 AM
To: Reuel Cornelia; Histonet
Subject: [Histonet] RE: Substitute Xylene for Bone tissue


I personally do not use any xylenes substitute for any of my undemineralized bone/resin histology processing. However, I have heard mention that several people use the traditional xylenes substitutes in conjunction with at least one change of fresh xylenes and some even substitute xylenes with methyl salicylate (oil of wintergreen).
 
In the past I would hear people complain that the xylenes substitutes did not do as good a job as regular xylenes, mostly due to inadequate removal of fat and especially in the larger animals with fatty marrow cavities, but maybe the xylenes substitutes have been optimized since then?
 
Jack
 



Date: Thu, 13 Oct 2011 11:13:05 -0500
From: Reuel.Cornelia <@t> tsrh.org
To: ratliffjack <@t> hotmail.com; histonet <@t> lists.utsouthwestern.edu
Subject: Substitute Xylene for Bone tissue



Does the Xylene substitute work very well with large bone tissue (5 to 7 cm long and between 3-5 mm thickness) for MMA and routine processing. What will be out pitfalls? We have a staff in our lab who is so "sensitive" to the smell of Xylene as she wanted to get pregnant sometime and the little smell that we have in our lab makes her emotional about it. Our lab has a very good ventilation but you could not prevent the smell when you open a retort after processing or even wiping small residue and placing it in our biohazard bin or simply cover slipping using xylene base mountant. I was planning to try the substitute to help make our environment  a better place for everyone.  Your helpful advise is always appreciated.
 
 
 
 Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768





***************************************************************************
Texas Scottish Rite Hospital for Children is one of the nation's leading
pediatric centers for the treatment of orthopedic conditions, certain 
related neurological disorders and learning disorders, such as dyslexia. 
This email transmission and/or its attachments may contain confidential 
health information, intended only for the use of the individual or entity
named above. 

The authorized recipient of this information is prohibited from disclosing
it to any other party unless required to do so by law and is required to
delete/destroy the information after its stated need has been fulfilled.
If you are not the intended recipient, any disclosure, copying,
distribution or action taken in reliance on the contents of this email
transmission is prohibited. If you have received this information in error,
please notify the sender immediately and delete this information. 

We appreciate your efforts to protect the children's confidential
information. 
*************************************************************************** 		 	   		  _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..