[Histonet] RE: Substitute Xylene for Bone tissue

[Jack Ratliff]

I personally do not use any xylenes substitute for any of my undemineralized bone/resin histology processing. However, I have heard mention that several people use the traditional xylenes substitutes in conjunction with at least one change of fresh xylenes and some even substitute xylenes with methyl salicylate (oil of wintergreen).
 
In the past I would hear people complain that the xylenes substitutes did not do as good a job as regular xylenes, mostly due to inadequate removal of fat and especially in the larger animals with fatty marrow cavities, but maybe the xylenes substitutes have been optimized since then?
 
Jack
 



Date: Thu, 13 Oct 2011 11:13:05 -0500
From: Reuel.Cornelia <@t> tsrh.org
To: ratliffjack <@t> hotmail.com; histonet <@t> lists.utsouthwestern.edu
Subject: Substitute Xylene for Bone tissue



Does the Xylene substitute work very well with large bone tissue (5 to 7 cm long and between 3-5 mm thickness) for MMA and routine processing. What will be out pitfalls? We have a staff in our lab who is so "sensitive" to the smell of Xylene as she wanted to get pregnant sometime and the little smell that we have in our lab makes her emotional about it. Our lab has a very good ventilation but you could not prevent the smell when you open a retort after processing or even wiping small residue and placing it in our biohazard bin or simply cover slipping using xylene base mountant. I was planning to try the substitute to help make our environment  a better place for everyone.  Your helpful advise is always appreciated.
 
 
 
 Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768





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