[Histonet] Chameleon tissue lifting off

[WILLIAM DESALVO]

I worked w/ animal tissue, not specifically lizard, several years ago and we always used Poly-L-Lysine coated slides. I found the polycationic nature of this molecule seemed to interact well w/ non-human species and created a strong interaction with the anionic sites of animal tissue sections. Poly-L-Lysine always produced strong adhesive properties. We also dried the sections at room temperature for 24 hours as we found that longer and slower worked best for us.

William DeSalvo, B.S., HTL(ASCP)

 

> From: nto <@t> stowers.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 30 Nov 2011 09:00:49 -0600
> Subject: [Histonet] Chameleon tissue lifting off
> 
> I would like to ask anyone who sections chameleon tissue what type of slides they use. I was having too much lifting while using the superfrost plus slides. It looked like slides coated with Haupt's solution were highly recommended, so I tried that. It is so much better, but still there is some lifting. If someone is successful with sectioning and staining lizard tissue without lifting, please advise me on type of slides, drying times, or anything that might help.
> Thank you so much,
> 
> Nancy Thomas
> Stowers Institute for Medical Research
> Kansas City, MO
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