[Histonet] Cutting mouse skin sections

Vagisha R vagishar <@t> gmail.com
Fri Nov 25 22:02:07 CST 2011


I've been trying to do hair follicle analysis in mouse skin sections for
the past couple of months.I usually fix the skin in Neutral Buffer
Formaline (NBF) for 24-48 hours and then send it to our histology
department to be embedded in paraffin blocks. However,i'm repeatedly
getting cross sections and broken hair follicles in my skin sections.Ive
tried embedding skin supported on paper and cutting the blocks at various
angles but it didn't make too much of a difference.If anything the stupid
paper is ruining the cutting blades!
Ive also tried cryosectioning snap frozen skin samples but that isn't
working out very well either.Does anyone have any clue at all why this is
happening and what i could possibly do to improve the section quality?


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