[Histonet] is there a way to recover ruined tissues

[Patsy Ruegg]

Please elaborate on this method.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: MARY T HODGES [mailto:hodges420 <@t> msn.com] 
Sent: Friday, November 25, 2011 7:04 PM
To: Rene J Buesa ; histonet <@t> lists.utsouthwestern.edu ; pruegg <@t> ihctech.net 
Subject: Re: [Histonet] is there a way to recover ruined tissues

Wrong all most complete recovery can be made with phenol 95 and water over
24 hours
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Date: Tue, 15 Nov 2011 21:14:20 
To: <histonet <@t> lists.utsouthwestern.edu>; <pruegg <@t> ihctech.net>
Subject: Re: [Histonet] is there a way to recover ruined tissues

Realy recovered? No!
 René J.
 
 --- On Tue, 11/15/11, Patsy Ruegg <pruegg <@t> ihctech.net> wrote:
 
 
 From: Patsy Ruegg <pruegg <@t> ihctech.net>
 Subject: [Histonet] is there a way to recover ruined tissues
 To: "'histo net'" <histonet <@t> lists.utsouthwestern.edu>
 Date: Tuesday, November 15, 2011, 3:26 PM
 
 
 My tissue processor was loaded and although the technician thought they
 started it, the next morning the tissues were found dry in the retort with
 the processor not started.  Another technician just started the machine so
 the tissues got processed as they would have been had it started the day
 before.  The tissues look terrible, they shrank so much that the pieces
 started coming out of the cassettes even though they were plenty large
 enough in the first place.  The tissues were found to be very small and
hard
 but were embedded, sectioned and stained anyway (I was not informed about
 this until I started questioning why the tissues looked so bad).
 
 
 
 Of course the investigator is very upset that their tissues are ruined.
 Does anyone know if there is any way to try and recovery these tissues?
 They were mouse lungs and livers.  The livers are not as bad as the lungs,
 the lungs were inflated and they look compressed and unrecognizable as lung
 tissue.  If the investigator will ever speak to me again I am going to ask
 for the blocks back and try melting them down and perhaps try to rehydrate
 them somehow for reprocessing.
 
 
 
 Regards,
 
 
 
 Patsy
 
 
 
 Patsy Ruegg, HT(ASCP)QIHC
 
 IHCtech
 
 12635 Montview Blvd. Ste.215
 
 Aurora, CO 80045
 
 720-859-4060
 
 fax 720-859-4110
 
 www.ihctech.net <http://www.ihctech.net>  
 
 www.ihcrg.org <http://www.ihcrg.org> 
 
 
 
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