[Histonet] Cutting mouse skin sections
jkiernan <@t> uwo.ca
Fri Nov 25 23:03:37 CST 2011
You need the advice of an experienced technician. Probably you will be advised to remove the paper after fixation and before trying to obtain either paraffin or cryostat sections. You have a histology department that processes your fixed tissues. Ask their technicians for advice.
Another word of advice from me: Say who and where you are. Do not hide behind a gmail address and expect to get good, free answers to your questions on Histonet or any other forum.
= = =
On 25/11/11, Vagisha R <vagishar <@t> gmail.com> wrote:
> I've been trying to do hair follicle analysis in mouse skin sections for
> the past couple of months.I usually fix the skin in Neutral Buffer
> Formaline (NBF) for 24-48 hours and then send it to our histology
> department to be embedded in paraffin blocks. However,i'm repeatedly
> getting cross sections and broken hair follicles in my skin sections.Ive
> tried embedding skin supported on paper and cutting the blocks at various
> angles but it didn't make too much of a difference.If anything the stupid
> paper is ruining the cutting blades!
> Ive also tried cryosectioning snap frozen skin samples but that isn't
> working out very well either.Does anyone have any clue at all why this is
> happening and what i could possibly do to improve the section quality?
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