[Histonet] RE: processing program for biopsies

pruegg <@t> ihctech.net pruegg <@t> ihctech.net
Sat Nov 5 21:24:59 CDT 2011

   i  agree  with Liz,  the  fat  in  the  skin requires a little   processing  than  other soft tissues especially human, animals d   have  as  much  fat  and should be processed on a shorter schedule to   keep them from becoming too hard and dehydrated.




   --------   Subject: [Histonet] RE: processing program fo   From: Elizabeth Chlipala <[1]liz <@t> premierlab.com>
   Date:   Fri,   November   04,   2011   10:37   amTo:   'Carol  Bryant'
   <[2]cbrya <@t> lexclin   "[3]hist   <[4]histonet <@t> lists.utsouthwestern.edu>
   In  my per   know  that skin b   longer  processing  cycle   minutes  to  1 hour per station for    cut just fine.
   Elizabeth   Manager
   Premier Laboratory, LLC
   PO   Boulder, CO 80308-1592
   (303) 682-3949 office
   (303) 682-   (303) 881-0763 cell
   [5]ww   Ship to address:
   1567 Skyway Drive, Unit   Longmont, CO 80504
   -----Original Message-----
   From:          [6]histonet-bounces <@t> list   [[7]mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  On  Behalf   Carol Bryant
   Sent: Friday, November 04, 2011 10:51 AM
   To: [8]histonet <@t> lists.utsouthwestern   Subject: [Histonet] processing program for biopsies
   Importan   Would  you  experienced  histotechs mind to look at this progr   see  if it would cause skin biopsies to be poorly processed? I think    too much time in alcohols.
   The pathologists say they look bad.
   Thanks   Station Solution Time Temp P/V Mix
   ---   1 Formalin 0:20 OFF   2 Formalin 0:20 OFF P/V Slow
   3 70% Alcohol 0:15 OFF P/V Slo   4 80% Alcohol 0:15 OFF P/V Slow
   5 95% Alcohol 0:15 OFF P/V Slow
      7 100% Alcohol 0:25 OFF P/V Slow
   8 10   9 Xylene 0:35 OFF P/V Slow
   10 Xylene 0:3   11 Paraffin 0:15 63C. P/V Slow
   12 Paraffin 0:15 63C. P   13 Paraffin 0:15 63C. P/V Slow
   14 Paraffin 0:15 63C. P/V Slow   Carol Bryant, CT (ASCP)
   Cytology/Histology Manager
   Lexing   Phone (859) 258-4082
   Fax (859) 258-4081
   [9]cbrya <@t> lexclin.com
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