[Histonet] RE: processing program for biopsies

[Kim Donadio]

I agree with Liz, its too short for big skins. I always call skins "chewy" because thats what they will be if not processed enough. 
 
In my experiance with this, skins that are small 1-2mm punch biopsies and shaves have done ok in a 15 min run for each with 30 min each for the formalins. Simular to what Carol showed here. 
 
Ask the Pathologist exactly what do they mean they look funny? washed out? etc because it could be the stain. Have you changed to any new reagents recently? 
 
Kim


________________________________
From: Elizabeth Chlipala <liz <@t> premierlab.com>
To: 'Carol Bryant' <cbrya <@t> lexclin.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, November 4, 2011 1:37 PM
Subject: [Histonet] RE: processing program for biopsies

In my personal opinion I think the processing cycle is too short.  I know that skin biopsies are tiny but we process all skin samples on longer processing cycles.  Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Friday, November 04, 2011 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing program for biopsies
Importance: High

Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols.
  The pathologists say they look bad.
Thanks in advance for your help!

  Station  Solution                Time  Temp  P/V    Mix
  -------  ----------------------  -----  -----  -----  ------
      1    Formalin                0:20  OFF  P/V    Slow
      2    Formalin                0:20  OFF  P/V    Slow
      3    70% Alcohol              0:15  OFF  P/V    Slow
      4    80% Alcohol              0:15  OFF  P/V    Slow
      5    95% Alcohol              0:15  OFF  P/V    Slow
      6    95% Alcohol              0:25  OFF  P/V    Slow
      7    100% Alcohol            0:25  OFF  P/V    Slow
      8    100% Alcohol            0:25  OFF  P/V    Slow
      9    Xylene                  0:35  OFF  P/V    Slow
      10    Xylene                  0:35  OFF  P/V    Slow
      11    Paraffin                0:15  63C.  P/V    Slow
      12    Paraffin                0:15  63C.  P/V    Slow
      13    Paraffin                0:15  63C.  P/V    Slow
      14    Paraffin                0:15  63C.  P/V    Slow


Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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