[Histonet] RE: processing program for biopsies
Blazek, Linda
lblazek <@t> digestivespecialists.com
Fri Nov 4 12:48:42 CDT 2011
What does look bad mean? In what way do they look bad? Do they cut well? What tissue processor do you use?
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Friday, November 04, 2011 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing program for biopsies
Importance: High
Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols.
The pathologists say they look bad.
Thanks in advance for your help!
Station Solution Time Temp P/V Mix
------- ---------------------- ----- ----- ----- ------
1 Formalin 0:20 OFF P/V Slow
2 Formalin 0:20 OFF P/V Slow
3 70% Alcohol 0:15 OFF P/V Slow
4 80% Alcohol 0:15 OFF P/V Slow
5 95% Alcohol 0:15 OFF P/V Slow
6 95% Alcohol 0:25 OFF P/V Slow
7 100% Alcohol 0:25 OFF P/V Slow
8 100% Alcohol 0:25 OFF P/V Slow
9 Xylene 0:35 OFF P/V Slow
10 Xylene 0:35 OFF P/V Slow
11 Paraffin 0:15 63C. P/V Slow
12 Paraffin 0:15 63C. P/V Slow
13 Paraffin 0:15 63C. P/V Slow
14 Paraffin 0:15 63C. P/V Slow
Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com
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