[Histonet] RE: processing program for biopsies

Elizabeth Chlipala liz <@t> premierlab.com
Fri Nov 4 12:37:55 CDT 2011

In my personal opinion I think the processing cycle is too short.  I know that skin biopsies are tiny but we process all skin samples on longer processing cycles.  Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Friday, November 04, 2011 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing program for biopsies
Importance: High

Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols.
  The pathologists say they look bad.
Thanks in advance for your help!

   Station  Solution                Time   Temp   P/V    Mix
   -------  ----------------------  -----  -----  -----  ------
       1    Formalin                 0:20   OFF   P/V    Slow
       2    Formalin                 0:20   OFF   P/V    Slow
       3    70% Alcohol              0:15   OFF   P/V    Slow
       4    80% Alcohol              0:15   OFF   P/V    Slow
       5    95% Alcohol              0:15   OFF   P/V    Slow
       6    95% Alcohol              0:25   OFF   P/V    Slow
       7    100% Alcohol             0:25   OFF   P/V    Slow
       8    100% Alcohol             0:25   OFF   P/V    Slow
       9    Xylene                   0:35   OFF   P/V    Slow
      10    Xylene                   0:35   OFF   P/V    Slow
      11    Paraffin                 0:15   63C.  P/V    Slow
      12    Paraffin                 0:15   63C.  P/V    Slow
      13    Paraffin                 0:15   63C.  P/V    Slow
      14    Paraffin                 0:15   63C.  P/V    Slow

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com


This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.  If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.  Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list