[Histonet] RE: processing program for biopsies

[Elizabeth Chlipala]

In my personal opinion I think the processing cycle is too short.  I know that skin biopsies are tiny but we process all skin samples on longer processing cycles.  Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Friday, November 04, 2011 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing program for biopsies
Importance: High

Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols.
  The pathologists say they look bad.
Thanks in advance for your help!

   Station  Solution                Time   Temp   P/V    Mix
   -------  ----------------------  -----  -----  -----  ------
       1    Formalin                 0:20   OFF   P/V    Slow
       2    Formalin                 0:20   OFF   P/V    Slow
       3    70% Alcohol              0:15   OFF   P/V    Slow
       4    80% Alcohol              0:15   OFF   P/V    Slow
       5    95% Alcohol              0:15   OFF   P/V    Slow
       6    95% Alcohol              0:25   OFF   P/V    Slow
       7    100% Alcohol             0:25   OFF   P/V    Slow
       8    100% Alcohol             0:25   OFF   P/V    Slow
       9    Xylene                   0:35   OFF   P/V    Slow
      10    Xylene                   0:35   OFF   P/V    Slow
      11    Paraffin                 0:15   63C.  P/V    Slow
      12    Paraffin                 0:15   63C.  P/V    Slow
      13    Paraffin                 0:15   63C.  P/V    Slow
      14    Paraffin                 0:15   63C.  P/V    Slow


Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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