[Histonet] Formic Acid Recipe

[Jack Ratliff]

Hello Jesus!
 
Try Sanderson's Rapid Bone Stain from Dorn and Hart Microedge (www.dornandhart.com). This is a metachromatic stain and should help you to penetrate the resin if heated at 60C and stained for 5-10 minutes depending upon section thickness. Rinse the slide in dH2O @ 60C and blot dry gently. Stain longer or shorter as necessary.
 
Jack

 

> From: jesus.w.hdz <@t> gmail.com
> Date: Fri, 4 Nov 2011 16:41:20 -0500
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Formic Acid Recipe
> 
> Dear all,
> 
> I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were loaded on hydroxyapatite scaffolds. I have also tried aniline blue and villaneuva stain. Any information on how I can make a solution of formic acid is appreciated along with maybe other stains I could try using. Each sample was grinded to about 50 microns. Thank you.
> 
> Best Regards,
> 
> Jesus W. Hernandez
> Graduate Student
> Department of Biomedical Engineering
> University of Texas at San Antonio
> jesus.w.hdz <@t> gmail.com
> 
> 
> 
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