[Histonet] Formic Acid Recipe

[Jesus Hernandez]

Dear all,

I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were loaded on hydroxyapatite scaffolds. I have also tried aniline blue and villaneuva stain. Any information on how I can make a solution of formic acid is appreciated along with maybe other stains I could try using. Each sample was grinded to about 50 microns. Thank you.

Best Regards,

 Jesus W. Hernandez
Graduate Student
Department of Biomedical Engineering
University of Texas at San Antonio
jesus.w.hdz <@t> gmail.com