[Histonet] RE: processing program for biopsies

[Tony Henwood]

Only 40 minutes in formalin is a problem.

Increase the formalin time to several hours and this should suffice for small 2-3mm punch biopsies of skin.

For larger skin sections the alcohol, xylene and wax times will need to be tripled at least (as well as fixing the skins for longer (3-4 hours on the machine would be great)).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Saturday, 5 November 2011 3:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing program for biopsies
Importance: High

Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols.
  The pathologists say they look bad.
Thanks in advance for your help!

   Station  Solution                Time   Temp   P/V    Mix
   -------  ----------------------  -----  -----  -----  ------
       1    Formalin                 0:20   OFF   P/V    Slow
       2    Formalin                 0:20   OFF   P/V    Slow
       3    70% Alcohol              0:15   OFF   P/V    Slow
       4    80% Alcohol              0:15   OFF   P/V    Slow
       5    95% Alcohol              0:15   OFF   P/V    Slow
       6    95% Alcohol              0:25   OFF   P/V    Slow
       7    100% Alcohol             0:25   OFF   P/V    Slow
       8    100% Alcohol             0:25   OFF   P/V    Slow
       9    Xylene                   0:35   OFF   P/V    Slow
      10    Xylene                   0:35   OFF   P/V    Slow
      11    Paraffin                 0:15   63C.  P/V    Slow
      12    Paraffin                 0:15   63C.  P/V    Slow
      13    Paraffin                 0:15   63C.  P/V    Slow
      14    Paraffin                 0:15   63C.  P/V    Slow


Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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