[Histonet] mast cells for arteries

[monettes <@t> mskcc.org]

Giemsa is what we use routinely for mast cell neoplasms and normal mast cells. For us it works equally well as T blue for diagnostic purposes. Our positive control is a cutaneous mast cell tumor (very common in out animal species), or skin biopsy with allergic dermatitis. 

Poorly granulated/degranulated mast cells won't stain with Giemsa or T Blue, and in those cases we use c-Kit IHC	

Best, 
Sebastien

Sébastien Monette, DMV, MVSc, DACVP
Tri-Institutional Laboratory of Comparative Pathology
Center of Comparative Medicine and Pathology
Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College
The Rockefeller University

1275 York Avenue, Box 270, New York NY 10065
Phone: 646-888-2420
Fax: 646-422-0139
Email MSKCC: monettes <@t> mskcc.org
Email WCMC: sem2010 <@t> med.cornell.edu
Web site: http://www.mskcc.org/mskcc/html/92361.cfm



-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Tuesday, November 01, 2011 1:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] mast cells for arteries

Does anyone have a go to stain for Mast Cells besides Tol Blue?  We are
trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining,
we used NH Skin and got maybe a few mast cells but I would like to use
something more abundant with mast cells.

 

Best regards,

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site  <http://www.ihctech.net> www.ihctech.net

 


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