[Histonet] mast cells for arteries

Patrick Laurie foreightl <@t> gmail.com
Tue Nov 1 13:23:56 CDT 2011

A good one that I used to use was the Naphthol AS-D Chloroacetate-esterase
which stains the non specific esterase activity in mast cells.  It is a
pain to run, but it is one of the most beautiful.  A bright red mast cell
against a pale background.  We got it from Vacca, p 530-533.

On Tue, Nov 1, 2011 at 10:07 AM, Patsy Ruegg <pruegg <@t> ihctech.net> wrote:

> Does anyone have a go to stain for Mast Cells besides Tol Blue?  We are
> trying to demonstrate the presence of mast cells in human arteries ffpe.
> Also, what would be a good positive control to use for mast cell staining,
> we used NH Skin and got maybe a few mast cells but I would like to use
> something more abundant with mast cells.
> Best regards,
> Patsy
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
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Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com

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