[Histonet] mast cells for arteries

Rosie Scrimizzi rosie_scrimizzi <@t> hotmail.com
Wed Nov 2 15:14:23 CDT 2011

Breast fibroadenoma is a great mast cell control
Rosie Scrimizzi

Sent from my iPad

On 03/11/2011, at 1:27 AM, monettes <@t> mskcc.org wrote:

> Giemsa is what we use routinely for mast cell neoplasms and normal mast cells. For us it works equally well as T blue for diagnostic purposes. Our positive control is a cutaneous mast cell tumor (very common in out animal species), or skin biopsy with allergic dermatitis. 
> Poorly granulated/degranulated mast cells won't stain with Giemsa or T Blue, and in those cases we use c-Kit IHC    
> Best, 
> Sebastien
> Sébastien Monette, DMV, MVSc, DACVP
> Tri-Institutional Laboratory of Comparative Pathology
> Center of Comparative Medicine and Pathology
> Memorial Sloan-Kettering Cancer Center
> Weill Cornell Medical College
> The Rockefeller University
> 1275 York Avenue, Box 270, New York NY 10065
> Phone: 646-888-2420
> Fax: 646-422-0139
> Email MSKCC: monettes <@t> mskcc.org
> Email WCMC: sem2010 <@t> med.cornell.edu
> Web site: http://www.mskcc.org/mskcc/html/92361.cfm
> -----Original Message-----
> From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
> Sent: Tuesday, November 01, 2011 1:08 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] mast cells for arteries
> Does anyone have a go to stain for Mast Cells besides Tol Blue?  We are
> trying to demonstrate the presence of mast cells in human arteries ffpe.
> Also, what would be a good positive control to use for mast cell staining,
> we used NH Skin and got maybe a few mast cells but I would like to use
> something more abundant with mast cells.
> Best regards,
> Patsy
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
> web site  <http://www.ihctech.net> www.ihctech.net
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