[Histonet] mast cells for arteries
llewllew <@t> shaw.ca
Tue Nov 1 12:25:02 CDT 2011
Go to http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm for a
reliable method. It still uses toluidine blue, but the staining is not
based on metachromasia and mast cell granules are dark blue. Most
toluidine blue samples will work, but occasionally one doesn't. Fisher
brand is OK as are many others.
I used to use a section from an acute appendicitis case as the control.
Patsy Ruegg wrote:
> Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
> trying to demonstrate the presence of mast cells in human arteries ffpe.
> Also, what would be a good positive control to use for mast cell staining,
> we used NH Skin and got maybe a few mast cells but I would like to use
> something more abundant with mast cells.
> Best regards,
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> wk email<mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
> web site<http://www.ihctech.net> www.ihctech.net
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