[Histonet] EM purchase
madary <@t> verizon.net
madary <@t> verizon.net
Tue May 24 13:47:59 CDT 2011
Fortunate enough to purchase and use several TEM and SEM's over the
ye= ars, I always found Zeiss to have the best prices and products for
TEM and = Hitachi for the SEM's. Last one I bought was 200K plus.
Good Luck and= let me know if you know of anyone looking for an EM
tech in the dc area.
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
Joni Madary, PhD(in life)May 2= 4, 2011 12:01:20 PM,
histonet <@t> lists.utsouthwestern.edu wrote:
Send Histonet= mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To= subscribe or unsubscribe via the World Wide Web, visit
http://lists.uts= outhwestern.edu/mailman/listinfo/histonet
or, via email, send a message = with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu=
You can reach the person managing the list at
histonet-owner <@t> lis= ts.utsouthwestern.edu
When replying, please edit your Subject line s= o it is more
specific
than "Re: Contents of Histonet digest..."
<= BR>Today's Topics:
1. RE: p63 (McMahon, Loralee A)
2. Re: Histone= t Digest, Vol 90, Issue 28 (boneslides <@t> aol.com)
3. RE: p63 (Bell, Lynne)=
4. Re: Freezing bath solutions (Robert Richmond)
5. Jones stain boo = boo (Norton, Sally)
6. Re: Re: Freezing bath solutions (mwhite <@t> mcleodhea= lth.org)
7. IHC Control Tissues (Goins, Tresa)
8. FISH (wassan alkadh= umi)
9. AW: [Histonet] FISH (Gudrun Lang)
10. Las Vegas Position (Hal= e, Meredith)
11. RE: p63 (Dessoye, Michael J)
12. Thank you (Margaret= Blount)
13. Re: Re: Freezing bath solutions (abright <@t> brightinstruments.=
com)
14. decal (Cynthia Pyse)
15. Re: decal (Rene J Buesa)
16. Pro= cessor power supply Back-up (kristen arvidson)
17. Coronavirus antibody = (Chakib Boussahmain)
18. Pathology Transmission Electron Micrscope (Vick= roy, Jim)
------------------------------------------------------=
----------------
Message: 1
Date: Mon, 23 May 2011 13:33:27 -0400=
From: "McMahon, Loralee A"
Subj= ect: [Histonet] RE: p63
To: Joanne Clark ,
"histonet= @lists.utsouthwestern.edu"
Messag= e-ID:
Content-Type: text/plain; charset=3D"us-ascii"
Bioca= re Medical
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Super= visor
Strong Memorial Hospital
Department of Surgical Pathology
(5= 85) 275-7210
________________________________________
From: histonet-= bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu= ] On Behalf Of Joanne
Clark [jclark <@t> pcnm.com]
Sent: Monday, May 23, 2011= 12:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] p= 63
I need to find a source for p63 antibody. Where does everyone pur=
chase theirs from?
Joanne Clark,HT
Pathology Consultants of New M= exico
_______________________________________________
Histonet ma= iling list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwest= ern.edu/mailman/listinfo/histonet
------------------------------=
Message: 2
Date: Mon, 23 May 2011 13:41:36 -0400 (EDT)
From: = boneslides <@t> aol.com
Subject: [Histonet] Re: Histonet Digest, Vol 90, Issu= e 28
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CDE787CC3=
64FAB-17A0-7F431 <@t> webmail-d058.sysops.aol.com>
Content-Type: text/plai= n; charset=3D"utf-8"
Dear Miss Margaret Blount:
Congratulatio= ns on your retirement!! May you enjoy good health
and many years doing the = things you WANT to do!!
God Bless!
-----Origi= nal Message-----
From: histonet-request
To: histonet
Sent: Mo= n, May 23, 2011 1:00 pm
Subject: Histonet Digest, Vol 90, Issue 28
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwe= stern.edu
To subscribe or unsubscribe via the World Wide Web, visit
h= ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
r, via email, s= end a message with subject or body 'help' to
histonet-request <@t> lists.utso= uthwestern.edu
You can reach the person managing the list at
histonet= -owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject = line so it is more
specific
han "Re: Contents of Histonet digest..."
=
oday's Topics:
1. Immuno controls (Carrie Disbrow)
2. This is goo= dbye (Margaret Blount)
3. In vitro mouse embryo culture (Carmen Maria Ga= rcia Pascual)
4. Re: Immuno controls (Rene J Buesa)
5. RE: Histonet D= igest, Vol 90, Issue 27 (Barone, Carol )
6. RE: Histonet Digest, Vol 90,= Issue 27 (Barone, Carol )
7. Freezing bath solutions (Scott, Allison D)=
8. p63 (Joanne Clark)
------------------------------------------=
---------------------------
Message: 1
ate: Sun, 22 May 2011 22:00:05= -0400
rom: Carrie Disbrow
ubject: [Histonet] Immu= no controls
o:
essage-ID:
ontent-Type: text/plain; charset= =3D"iso-8859-1"
i all,
fter consideration I disagree with the= consensus and also agree
with Curt's
athologist. More supplies and lab= or will be involved as cutting
more controls
ill be neccessay to meet t= he requests of the pathologist. The
patient tissue is
ut on two control= s slides. One slide is used as the negative
control and has
oth the pat= ient tissue and the known positive control which
should both be
egative= after the run. The other slide with the known positive
control and the
atient tissue in question is also stained with the positive control
staini= ng
ositive.
hank you!
------------------------------
Messa= ge: 2
ate: Mon, 23 May 2011 09:04:54 +0100
rom: "Margaret Blount"
ubject: [Histonet] This is goodbye
o:
essage-ID:
ontent-Type: text/plain; charset= =3D"US-ASCII"
To everyone in histoland,
This is my last full week= at work. From next Tuesday I will be
retiring
rom my job at Cambridge U= niversity. I have thoroughly enjoyed my
chosen
rofession as a research t= echnician specialising in histology. I
realise
hat because I have always= worked in research my experience is
somewhat
imited: I haven't been ask= ed for the same range of techniques
that you
ind in a routine clinical l= ab. However, I have enjoyed my work
and have
earnt and devised some tech= niques for a range of interesting
samples.
ow it's time to learn somethi= ng new!
I hope to spend more time with my lace pillow and learn to = do it
roperly, maybe learn to do Northamptonshire lace which I believe i=
s a
ariation on Bucks point. (I come originally from Northants) I have
h= ad
go at Bedfordshire and will learn more, it is very pretty. I also
pla= n
o travel; my next trip is to New Zealand in November.
I won't = go on. I just want to say this: You are all great guys
and
irls; you sha= re your experience and friendship with great
generosity,
hich I have alw= ays felt is what a scientist should do. Thank you
for
our comradeship an= d help over the years. Maybe I'll get to meet
some of
ou by chance durin= g my travels.
Best wishes to you all and a great big Thank You,
<= BR>From
Margaret
Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science<= BR>Box 289, Addenbrooke's
Hospital
Hills Road, Cambridge, CB2 0QQ
Tel 01223 769061/336079
------------------------------
Messa= ge: 3
ate: Mon, 23 May 2011 10:35:20 +0200 (CEST)
rom: "Carmen Maria = Garcia Pascual"
ubject: [Histonet] In vitro mouse= embryo culture
o: histonet <@t> lists.utsouthwestern.edu
essage-ID: <6= 395165382carmaga6 <@t> uv.es>
ontent-Type: text/plain; charset=3D"ISO-8859= -1"
Good Moorning to everyone:
I am Carmen Garcia a PhD student f= rom Valencia, we are know
interesting
n make in vitro culture of mouse = embryos to do toxicity tests and
in
ur lab we have not experience. some= one can give me some advise?
or
ecommend me some bibliography?
ow lo= ng can I have the embryos in culture?
hank you so much!!!
Best regard= s,
armen
------------------------------
Message: 4
ate:= Mon, 23 May 2011 07:48:50 -0700 (PDT)
rom: Rene J Buesa
ubject: Re: [Histonet] Immuno controls
o: Carrie Disbrow ,
"histonet <@t> lists.utsouthwestern.edu"
essage-ID: <87688.31423.qm <@t> web65714.mail.ac4.yahoo.co= m>
ontent-Type: text/plain; charset=3Diso-8859-1
Also Sprach Zarat= hustra!!!!
en=C3=AF=C2=BF=C2=BD J.
From: Carrie Disbrow
o: histonet <@t> lists.utsouthwestern.edu
ent: Sunday, May 22, 2011 = 10:00 PM
ubject: [Histonet] Immuno controls
i all,
fter co= nsideration I disagree with the consensus and also agree
with Curt's
at= hologist. More supplies and labor will be=C3=AF=C2=BF=C2=BD
involved as=C3= =AF=C2=BF=C2=BD cutting more controls
ill be neccessay to meet the requ= ests of the pathologist. The
patient tissue is
ut on two controls slide= s. One slide is used as the negative
control and has
oth the patient ti= ssue and the known positive control which
should both be
egative after = the run. The other slide with the known positive
control and the
atient= tissue in question is also stained with the positive
control staining
= ositive.
hank you!
=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF= =C2=BD
=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD
=C3=AF=C2=BF= =C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD
=C3=AF=C2=BF=C2=BD =C3=AF=C2=BF=
=C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD
=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF= =C2=BD=C3=AF=C2=BF=C2=BD
=C3=AF=C2=BF=C2=BD _______________________________=
________________
istonet mailing list
istonet <@t> lists.utsouthwestern.ed= u
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
----------= --------------------
Message: 5
ate: Mon, 23 May 2011 11:02:16 -0400<= BR>rom: "Barone, Carol "
ubject: [Histonet] RE: Hi= stonet Digest, Vol 90, Issue 27
o: essage-ID:
ontent-Type: text/plain; charset=3D"us-ascii"
-----Original Me= ssage-----
rom: histonet-bounces <@t> lists.utsouthwestern.edu
mailto:hist= onet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
istonet-request <@t> list= s.utsouthwestern.edu
ent: Sunday, May 22, 2011 1:01 PM
o: histonet <@t> li= sts.utsouthwestern.edu
ubject: Histonet Digest, Vol 90, Issue 27
Send= Histonet mailing list submissions to
To subscribe or unsubscribe vi= a the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/list= info/histonet
r, via email, send a message with subject or body 'help' t= o
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person = managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When rep= lying, please edit your Subject line so it is more
specific than
Re: Con= tents of Histonet digest..."
oday's Topics:
1. to Rene J Buesa (M= axim Peshkov)
------------------------------------------------------=
---------------
Message: 1
ate: Sun, 22 May 2011 13:43:07 +0400
ro= m: Maxim Peshkov
ubject: [Histonet] to Rene J Buesao: Rene J Buesa
c: histonet <@t> lists.utsouthwestern.e= du
essage-ID: <1585823684.20110522134307 <@t> mail.ru>
ontent-Type: = text/plain; charset=3Dwindows-1251
I am sorry, that uses histonet for pe= rsonal goals.
Dear Rene!
ast 3 my answers for you are returned back t= o me.
our e-mails I got successfully.
upport service of mail.ru wrote= that problem is at yahoo.com
server.
lease check your postmaster servic= e.
Here is my post-address:
axim Peshkov,
l. Mikhailovskaya, 90-A,=
47909,
aganrog,
ussia.
Sincerely,
axim.
mailto:Maxim_71@= mail.ru
-----------------------------
_______________________= ________________________
istonet mailing list
istonet <@t> lists.utsouthwe= stern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
En= d of Histonet Digest, Vol 90, Issue 27
*********************************= ******
------------------------------
Message: 6
ate: Mon, 23 = May 2011 11:15:34 -0400
rom: "Barone, Carol "
u= bject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27
o:
essage-ID:
ontent-Type: text/plain; charset=3D"us-ascii"=
Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct,
thatad already been in formailin two weeks (was FFPE). Normally
would do
n= a frozen 1:40? ....We have background everywhwere on first
un...suggest= ions? Neg is also shining??? Crazy researcher's! Got
to
ove them...when = will they learn to ask first?
We are diluting primary in plan two, and w= ill stain over-night at
4
egrees....but, this thing is lit-up like the S= UN!
was expecting low signal? Go figure?
----Original Message---= --
rom: histonet-bounces <@t> lists.utsouthwestern.edu
mailto:histonet-bou= nces <@t> lists.utsouthwestern.edu] On Behalf Of
istonet-request <@t> lists.utsout= hwestern.edu
ent: Sunday, May 22, 2011 1:01 PM
o: histonet <@t> lists.utso= uthwestern.edu
ubject: Histonet Digest, Vol 90, Issue 27
Send Histone= t mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To su= bscribe or unsubscribe via the World Wide Web, visit
http://lists.utsout= hwestern.edu/mailman/listinfo/histonet
r, via email, send a message with= subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
= You can reach the person managing the list at
histonet-owner <@t> lists.utsou= thwestern.edu
When replying, please edit your Subject line so it is more=
specific than
Re: Contents of Histonet digest..."
oday's Topics:=
1. to Rene J Buesa (Maxim Peshkov)
-----------------------------=
----------------------------------------
Message: 1
ate: Sun, 22 May = 2011 13:43:07 +0400
rom: Maxim Peshkov
ubject: [Hi= stonet] to Rene J Buesa
o: Rene J Buesa
c: histon= et <@t> lists.utsouthwestern.edu
essage-ID: <1585823684.20110522134307 <@t> mai= l.ru>
ontent-Type: text/plain; charset=3Dwindows-1251
I am sorry, = that uses histonet for personal goals.
Dear Rene!
ast 3 my answers fo= r you are returned back to me.
our e-mails I got successfully.
upport= service of mail.ru wrote that problem is at yahoo.com
server.
lease che= ck your postmaster service.
Here is my post-address:
axim Peshkov,
l. Mikhailovskaya, 90-A,
47909,
aganrog,
ussia.
Sincerely,
= axim.
mailto:Maxim_71 <@t> mail.ru
-----------------------------______________________________________
_________
istonet mailing lististonet <@t> lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailma= n/listinfo/histonet
End of Histonet Digest, Vol 90, Issue 27
********= *******************************
------------------------------
Me= ssage: 7
ate: Mon, 23 May 2011 11:22:24 -0500
rom: "Scott, Allison D"=
ubject: [Histonet] Freezing bath solutions=
o:
essage-ID:
<1872B4A455B= 7974391609AD8034C79FC026DFDD9 <@t> LBEXCH01.hchd.local>
ontent-Type: text/= plain; charset=3D"us-ascii"
Hello to all in histoland. What type of solu= tions are being used
if any
o freezze your chucks for frozen sections. O= ur sister hospital is
sing methyl butane and there was a flash fire last= week. We use
the
ame solution. Any suggestions will be greatly apprecia= ted.
Allison Scott HT(ASCP)
istology Supervisor
BJ Hospital
ONF= IDENTIALITY NOTICE:
f you have received this e-mail in error, please imm= ediately
notify the
ender by return e-mail and delete this e-mail and an= y attachments
from
our computer system.
To the extent the informatio= n in this e-mail and any attachments
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rotected health informatio= n as defined by the Health Insurance
Portability
nd Accountability Act = of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts
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64; or Chapter 181,= Texas Health and Safety Code, it is
confidential and/or
rivileged. Thi= s e-mail may also be confidential and/or privileged
under
exas law. The= e-mail is for the use of only the individual or
entity named
bove. If = you are not the intended recipient, or any authorized
epresentative of = the intended recipient, you are hereby notified
that any
eview, dissemi= nation or copying of this e-mail and its
attachments is
trictly prohibi= ted.
------------------------------
Message: 8
ate: Mon, 23 Ma= y 2011 16:31:08 +0000
rom: Joanne Clark
ubject: [Hi= stonet] p63
o: "histonet <@t> lists.utsouthwestern.edu"
essage-ID:
<0494A7D4E8CC254EA2FB81464982E3786DCA= 72 <@t> S10MAILD001N1.SH10.lan>
ontent-Type: text/plain; charset=3D"us-asc= ii"
I need to find a source for p63 antibody. Where does everyone
purcha= se theirs
rom?
Joanne Clark,HT
athology Consultants of New Mexico=
------------------------------
_________________________________= ______________
istonet mailing list
istonet <@t> lists.utsouthwestern.edu<=
BR>ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histo= net Digest, Vol 90, Issue 28
*
--------------------------= ----
Message: 3
Date: Mon, 23 May 2011 13:45:50 -0400
From: "B= ell, Lynne"
Subject: [Histonet] RE: p63
To: 'Jo= anne Clark' ,
"histonet <@t> lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/plain; char= set=3D"us-ascii"
I believe the only place you can buy p63 is through= Biocare,
www.biocare.net.
Lynne Bell, HT (ASCP)
Histology Te= am Leader
802-371-4923
------------------------------
=
Message: 4
Date: Mon, 23 May 2011 14:12:37 -0400
From: Robert Ric= hmond
Subject: [Histonet] Re: Freezing bath solut= ions
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset= =3DISO-8859-1
Allison Scott after a flash fire with the stuff asks a= bout
alternatives to 2-methylbutane for freezing specimens.
There= is indeed a safe alternative, though I haven't had a chance
to
try it. = I've posted about it on Histonet more than once - here's
a
copy of an ea= rlier post of mine:
3M(tm) Novec(tm) Engineered Fluid HFE-7100
Th= is product belongs to a class of fluorocarbons called
"segregated hydrof= luoroethers (HFE's)"
According to various MSDS, HFE-7100 is
methyl no= nafluoroisobutyl ether
C4F9-O-CH3
It melts and freezes at -135 C, so = that it would remain liquid in
the
Histobath. (It would freeze solid in = liquid nitrogen, however.)
It boils at 60 C., and is listed as non-flamm= able. It has almost
no
vapor pressure at freezing temperatures.
It co= st around $230 a gallon a few years ago.
Bob Richmond
Samurai Pat= hologist
Knoxville TN
------------------------------
<= BR>Message: 5
Date: Mon, 23 May 2011 11:28:28 -0700
From: "Norton, Sa= lly"
Subject: [Histonet] Jones stain= boo boo
To:
Message-ID:
<1= 6E0693C7018C245959AC729FE66EDE52DBECB <@t> s107.childrens.sea.kids>
Conten= t-Type: text/plain; charset=3D"us-ascii"
I accidentally put my renal= slides in the Methanimine Silver
(after
Periodic Acid) before putting t= hem in the Thiosemicarbizine.
Slides
take on a yellow hue which makes th= e H&E counterstain muddy. Is
there a
way to correct this?
Thank you,
Sally Norton
Histotech
CONFIDENTIALITY NOTICE: This e-mail message, including any attach=
ments, is for the sole use of the intended recipient(s) and may
contain con= fidential and privileged information protected by law.
Any unauthorized rev= iew, use, disclosure or distribution is
prohibited. If you are not the inte= nded recipient, please contact
the sender by reply e-mail and destroy all c= opies of the original
message.
-----------------------------= -
Message: 6
Date: Mon, 23 May 2011 14:46:29 -0400
From: mwhit= e <@t> mcleodhealth.org
Subject: Re: [Histonet] Re: Freezing bath solutionsTo: Robert
Richmond
Cc: histonet <@t> lists.utsouthw= estern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
=
Content-Type: text/plain; charset=3D"us-ascii"
Allison and = Dr. Richmond:
We are using Novec 7000 successfully in our freezing b= ath and
have been
doing so for about 6 months with no major problems.
It does have some annoying traits ; however the safety benefits
outwei= gh
those issues , in my opinion. The issues are: a little more time
requ= ired
for specimen to freeze, tissues tending to float in the solution,
a= nd
evaporation. I worried that the evaporation would be a big problem
, = but
it has not .My techs discovered through experimentation that by
lowe= ring
the tissue slowly into the fluid, it would stop the flotation
probl= em.
Overall, I would recommend it. 3M has been very helpful to us. (=
I'm not
being paid in any way to endorse this!!) See below for 3M contac= t
information.
Additionally, through earlier Histonet inquiries, = I was told
about a
freezing bath for those of you who still use the old = Histobath
that is no
longer made. If anyone wants information about it, = It's on
www.ftssystems.com and the item is called a "Mult-Cool Bath". We=
don't have
one , but it looks like it could be a suitable replacement.<= BR>
Here's our purchasing contact person at 3M:
Christina Cerecedes1-888-509-5224
Melanie S. White, MT(ASCP)
Laboratory Superv= isor, Systems/Anatomic Pathology
McLeod Regional Medical Center
Flore= nce, SC
(843) 777-2072
NOTICE: This e-mail message and al= l attachments transmitted with
it may contain legally PRIVILEGED and CO= NFIDENTIAL information
intended solely for the use of the addressee. If= the reader of
this
message is not the intended recipient, you are here= by notified
that any reading, dissemination, distribution, copying, or = other
use
of this message or its attachments is strictly prohibited. If= you
have received this message in error, please notify the sender
= immediately and/or notify the postmaster
(postmaster <@t> mcleodhealth.org), and= delete this message and all
copies and backups thereof. Thank You.
=
------------------------------
Message: 7
Date: Mon, = 23 May 2011 18:56:05 +0000
From: "Goins, Tresa"
Subje= ct: [Histonet] IHC Control Tissues
To: "histonet <@t> lists.utsouthwestern.ed= u"
Message-ID:
Content-Type: text/pla= in; charset=3D"us-ascii"
If you haven't made up your mind yet on thi= s issue, here are a
couple of references that may - or may not - help:
<= BR>Veterinary Diagnostics:
http://www.ncbi.nlm.nih.gov/sites/entrez?=
orig_db=3DPubMed&db=3Dpubmed&cmd=3DSearch&term=3D%22Journal%20o=
f%20veterinary%20diagnostic%20investigation%20%3A%20official%20publ
ication%=
20of%20the%20American%20Association%20of%20Veterinary%20Laboratory%
20Diagno=
sticians%2C%20Inc%22%5BJour%5D%20AND%2020%5Bvolume%5D%20AND%20393%5
Bpage%5D= %20AND%202008%5Bpdat%5D
FDA
F.20 Quality Control:
=
http://www.fda.gov/downloads/MedicalDevices/DeviceRegulationandGuid
ance/Gui= danceDocuments/ucm094015.pdf
Tresa Goins
Histopathology Super= visor
Department of Livestock
Bozeman, Montana
-------= -----------------------
Message: 8
Date: Mon, 23 May 2011 12:23:5= 4 -0700 (PDT)
From: wassan alkadhumi
Subject:= [Histonet] FISH
To: h n
Message-= ID: <539226.53686.qm <@t> web45202.mail.sp1.yahoo.com>
Content-Type: te= xt/plain; charset=3Diso-8859-1
Dear members
We jus= t started doing FISH using HER2 FISH pharma Dx Dako kit,
Code number K5331 =
on malignant breast tissues.
I was wondering what is the succe= ss ratio or percentage for say
a one run?? my
hospital is the firs= t in our region to start FISH
and I am the first to run it. = I have no experience with the
hybridizer but by
reading the&= nbsp;hybridizer manual and the kit insert i discover
it is user friendly. <= BR>I run the first batch (six slides
only) yesterday. Continue an= d finish it today.
only three of the slides worked.
I will star= t a new batch next week and I appreciate any help you
may offer to
avoi= d inaccurate result.
Thank you
Wassan
Histotec= hnician
Shorsh General Hospital
North of Iraq
----------------= --------------
Message: 9
Date: Mon, 23 May 2011 22:15:37 +0200From: "Gudrun Lang"
Subject: AW: [Histonet] FISH
T= o: "'wassan alkadhumi'"
Cc: histonet <@t> lists.utsou= thwestern.edu
Message-ID: <00DDDF450F674127ACFF7D694679751A <@t> dielangs.= at>
Hi Wassan,
you may depart the FISH procedure in a tissue-d= ependant first
part and a
probe-dependant second part.
The first part= comprises the pretreatment of the tissue to
retrieve and
permeabilises = it. The amount of pretreatment is dependant on the
fixation-duration of = the tissue.
The longer the fixation - the higher the temperature of the =
buffer, the
longer the incubation time in the buffer, the longer the inc=
ubation time in
the protease.
Troubleshooting in this part:
To= o low temperature of some slides because of inconsistent
temperature in
= the waterbath. Tissueslides mounted on the glass-slides in
different
hei= ghts.
Too short protease-incubation in relation to the fixation time. Us=
ually
nuclei can be digested until you see the first tiny holes. Underdi=
gested
nuclei are dense and look grey in the triplefilter.
If the nuc= lei are overdigested, they get bigger holes and fuzzy
borders.
Different= staining results due to variing fixation-times.
The second part= comprises the stringent wash. The temperature,
buffer-quality, duration= are probe-depending and are usually
provided in the
kit-handbook.
Troubleshooting second part:
Too high temp leads to loosing the bound = probe.
Too low temp leads to high background because of unspecifc bound =
probes.
With your problem I would try to prolong the digestion in 10= min
steps,
until a good result is found.
Hint: standardization of fi= xation is the crucial point. In our
lab the
tumor-block is immediately f= ixed in a cassette as a FISH-block.
So it will
be fixed very well and wi= thstands the harsh treatment. The
disadvantage is
stronger autofluoresce= nce.
A further hint: be sure, that the gum around the coverslip is
harde= ned
before starting the hybridizer-protocol. 10 min drying time.
= I hope this helps
Gudrun Lang
-----Urspr=C3=BCngliche= Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailt= o:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
wassan
alkad= humi
Gesendet: Montag, 23. Mai 2011 21:24
An: h n
Betreff: [Histon= et] FISH
Dear members
We just started doing FISH u= sing HER2 FISH pharma Dx Dako kit,
Code number
K5331
on malignant bre= ast tissues.
I was wondering what is the success ratio or percenta= ge for say
a one
run?? my
hospital is the first in our region&nb= sp;to start FISH
and I am the first to run it. I have no experien= ce with the
hybridizer
but by
reading the hybridizer = manual and the kit insert i discover it is
user
friendly.
I run the f= irst batch (six slides only) yesterday. Continue
and finish = it
today.
only three of the slides worked.
I will start a new batc= h next week and I appreciate any help you
may offer
to
avoid inaccura= te result.
Thank you
Wassan
Histotechnician
Shorsh General Hospital
North of Iraq
______________________________= _________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern= .edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mo= n, 23 May 2011 16:53:16 -0500
From: "Hale, Meredith" =
Subject: [Histonet] Las Vegas Position
To:
Message-ID:
<6F33D8418806044682A391273399860F08374FCA <@t> s=
-irv-ex301.PathologyPartners.intranet>
Content-Type: text/plain; = charset=3D"us-ascii"
Great opportunity for a Histotechnician= in a brand new laboratory!
We
are a 5 physician gastroenterology practi= ce located in Las Vegas,
NV
looking for a certified HT or HTL. Candidate= must meet CLIA
grossing
requirements. The candidate will be responsible= for routine
histology
duties. This is a part-time position that offers = a competitive
salary
and flexible hours. Interested applicants should co= ntact Meredith
Hale,
phone (214)596-2219 or e-mail mhale <@t> carisls.com
=
Meredith Hale HT (ASCP) CM
Operations Liaison Di= rector and Education Coordinator
Caris Life Sciences
6655 North MacArthur Blvd, Irving Texas 75039
direct: 214-596-2219<= BR>
cell: 469-648-8253
fax: 214-596-7095
mhale <@t> carisls.com=
=
------------------------------
Message: 11
Date: Tue,= 24 May 2011 07:14:23 -0400
From: "Dessoye, Michael J"
Subject: RE: [Histonet] p63
To: "Joanne Clark" = ,
Message-ID:
Content-Type: text/p= lain; charset=3D"iso-8859-1"
Biocare has a very good, and fairly ine= xpensive, p63 that we have
had good results with: http://biocare.net/produc=
ts/antibodies/p/163/
________________________________
From: J= oanne Clark [mailto:jclark <@t> pcnm.com]
Sent: Mon 5/23/2011 12:31 PM
To:= histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] p63
I need to find a source for p63 antibody. Where does everyone
purchase th= eirs from?
Joanne Clark,HT
Pathology Consultants of New Mexico
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ = _ _ _ _
_ _ _ _ _
This email and any files transmitted with it are c= onfidential and
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Any views expressed in this message are those of the individua= l
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------------------------------
Message: 12
Date: Tue= , 24 May 2011 12:14:02 +0100
From: "Margaret Blount"
Subject: [Histonet] Thank you
To:
Message-ID:
Content-Type: text/plain; charset=3D"US-ASCII"
Hello everyone!
Thank you all for your good wishes on my r= etirement. I knew you
were all
great people: you have just proved it big= time!
Thank you all from the bottom of my heart.
Keep smiling!
Much love
Margaret
=
------------------------------
Message: 13
Date: Tue, 24 = May 2011 13:03:21 +0000
From: abright <@t> brightinstruments.com
Subject: = Re: [Histonet] Re: Freezing bath solutions
To: mwhite <@t> mcleodhealth.org,<=
BR>histonet-bounces <@t> lists.utsouthwestern.edu, "Robert Richmond"
Cc: Histonet
Messa= ge-ID:
<757762693-1306242203-cardhu_decombobulator_blackberry.rim.net=
-1362851250- <@t> b15.c2.bise7.blackberry>
Content-Type: text/plain; c= harset=3D"Windows-1252"
Melanie,
In our Clini-RF rapid freezer we= have a device for keeping tissue
submerged in Novec 7000.
Regards <= BR>
Alan Bright
www.brightinstruments.com
Sent from my BlackBerry= =C2=AE wireless device
-----Original Message-----
From: mwhite <@t> mc= leodhealth.org
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date= : Mon, 23 May 2011 14:46:29
To: Robert RichmondCc: ;
Subject: Re: [Histonet] Re: Freezing bath solutions
A= llison and Dr. Richmond:
We are using Novec 7000 successfully in our= freezing bath and have
been
doing so for about 6 months with no major p= roblems.
It does have some annoying traits ; however the safety bene= fits
outweigh
those issues , in my opinion. The issues are: a little mor= e time
required
for specimen to freeze, tissues tending to float in the = solution,
and
evaporation. I worried that the evaporation would be a big= problem
, but
it has not .My techs discovered through experimentation t= hat by
lowering
the tissue slowly into the fluid, it would stop the flot= ation
problem.
Overall, I would recommend it. 3M has been very helpf= ul to us.
(I'm not
being paid in any way to endorse this!!) See below fo= r 3M contact
information.
Additionally, through earlier Histonet = inquiries, I was told
about a
freezing bath for those of you who still u= se the old Histobath
that is no
longer made. If anyone wants information= about it, It's on
www.ftssystems.com and the item is called a "Mult-Coo= l Bath". We
don't have
one , but it looks like it could be a suitable re= placement.
Here's our purchasing contact person at 3M:
Christina = Cerecedes
1-888-509-5224
Melanie S. White, MT(ASCP)
Labora= tory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Cent= er
Florence, SC
(843) 777-2072
NOTICE: This e-mail mes= sage and all attachments transmitted with
it may contain legally PRIVIL= EGED and CONFIDENTIAL information
intended solely for the use of the ad= dressee. If the reader of
this
message is not the intended recipient, y= ou are hereby notified
that any reading, dissemination, distribution, c= opying, or other
use
of this message or its attachments is strictly pro= hibited. If you
have received this message in error, please notify the = sender
immediately and/or notify the postmaster (postmaster <@t> mcleodhealt=
h.org), and delete this message and all copies and backups thereof.
Thank Y= ou.
_______________________________________________
Histonet = mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwe= stern.edu/mailman/listinfo/histonet
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Message: 14
Date: Tue, 24 May 2011 10:56:52 -0400
From: "= Cynthia Pyse"
Subject: [Histonet] decal
To: "'H= istonet'"
Message-ID: <001101cc1a= 22$d100e9d0$7302bd70$@com>
Content-Type: text/plain; charset=3D"us-as= cii"
Hello Histonetters
What is everyone using to decal speci= mens. I haven't had to use
it in a few
years, before that I just purchas= ed formic acid and made a 10%
solution.
Just wanted to see if the criter= ia has changes over the years.
Thanks in
advance for the responses.
<= BR>
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Superviso= r
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com
<= BR>
------------------------------
Message: 15
Dat= e: Tue, 24 May 2011 08:50:13 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] decal
To: Cynthia Pyse , 'Histonet'
Message-ID: &= lt;259132.9447.qm <@t> web65711.mail.ac4.yahoo.com>
Content-Type: text/pla= in; charset=3Diso-8859-1
For tissues containing bone, or bone specim= ens I always used the
commercial brand RDO.
For bone marrow biopsi= es I used EDTA (prepared in the lab).
Ren=C3=A9 J.
From: Cynthia = Pyse
To: 'Histonet'
Sent: Tuesday, May 24, 2011 10:56 AM
Subject: [Histonet] decal<= BR>
Hello Histonetters
What is everyone using to decal specimens.= I haven't had to use it
in a few
years, before that I just purchased fo= rmic acid and made a 10%
solution.
Just wanted to see if the criteria ha= s changes over the years.
Thanks in
advance for the responses.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
=
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com
_______________________________________________
Histonet mailing l= ist
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu= /mailman/listinfo/histonet
------------------------------
Mes= sage: 16
Date: Tue, 24 May 2011 09:01:03 -0700 (PDT)
From: kristen ar= vidson
Subject: [Histonet] Processor power s= upply Back-up
To: histonet
Messag= e-ID: <2613.13896.qm <@t> web65711.mail.ac4.yahoo.com>
Content-Type: te= xt/plain; charset=3Diso-8859-1
Does anyone have any suggestions on p= ower supply back-up for
Leica ASP 300? We are an independant lab and = are not hooked up
to back-up if we have an outage. Thought it might b= e a good idea
:)
Thanks.
------------------------------=
Message: 17
Date: Tue, 24 May 2011 09:34:55 -0700 (PDT)
From:= Chakib Boussahmain
Subject: [Histonet] Coronavirus= antibody
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <42506= 8.63659.qm <@t> web161818.mail.bf1.yahoo.com>
Content-Type: text/plain; ch= arset=3Dus-ascii
Hi Histonetters,
We are interesting in doing imm= unostain on ferret tissues that
have been fixed in formalin and embedded in= paraffin. I am just
wondering if anyone used this antibody in their lab, i= f so, can
you please share with us your staining protocol? antigen retrieva=
l protocol as well?
Your help will be very much appreciated.
Thank yo= u
Chakib HTL(ASCP)
Histology lab
MIT
----------------------= --------
Message: 18
Date: Tue, 24 May 2011 11:46:17 -0500
Fro= m: "Vickroy, Jim"
Subject: [Histonet] Pathology = Transmission Electron Micrscope
To: "histonet <@t> lists.utsouthwestern.edu"<= BR>
Message-ID:
<24A4826E8EF096=
4D86BC5317306F58A55DF5BEC1C4 <@t> mmc-mail.ad.mhsil.com>
Content-Type: tex= t/plain; charset=3D"us-ascii"
We are in the market to purchase a= new TEM. Our old Hitachi
instrument is 31 years old and is getting tired. = The use for
transmission em in pathology has somewhat diminished in our hos=
pital environment. We still however use TEM routinely on all renal
biopsies= and nerve biopsies. Has anyone priced TEM scopes for
pathology usage latel= y. In the past there have not been many
options for a TEM that was for path= ology usage verses one for
research. Can anyone steer me in the right direc= tion. Of course
cost has become a major issue also so we are trying to make= the
best choice for our usage.
Thanks
James Vickroy BS, HT(A= SCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial= Medical Center
217-788-4046
________________________________=
This message (including any attachments) contains confidential
informat= ion intended for a specific individual and purpose, and
is protected by law= . If you are not the intended recipient, you
should delete this message. An= y disclosure, copying, or
distribution of this message, or the taking of an= y action based
on it, is strictly prohibited.
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End of Histonet Digest, Vol= 90, Issue 29
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