[Histonet] Formalin fixed frozen sections

[Patsy Ruegg]

I fix frozen tissue in reg 10% NB formalin and then process it into paraffin
all the time.  I haven't tried it but if someone gave me some unfixed frozen
tissue (especially bone) and wanted me to do frozen tape sectioning I would
recommend that we thaw fix the original frozen block in formalin, then
infiltrate in sucrose and re snap freeze it as they would section better.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
amitapandey <@t> torrentpharma.com
Sent: Wednesday, May 18, 2011 10:02 PM
To: Sherwood, Margaret 
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin fixed frozen sections

We  take frozen section from formalin fixed tissue  and  placed them  in 
30% sucrose overnight before mounting on chucks.

But never tried the other way round  frozen tissue then fixed formalin, i 
heard that  it helps in falling of the tissue. But no experience.


Amita



From:   "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
To:     <histonet <@t> lists.utsouthwestern.edu>
Date:   19/05/11 01:21 AM
Subject:        Re:  [Histonet] Formalin fixed frozen sections
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



Does anyone fix their frozen tissue first in formalin before embedding and
sectioning?  I know one investigator in our lab did that previously, but 
was not
sure of the strength of formalin (I don't believe it was 10%).  Another
investigator inquired about doing that.

I would appreciate hearing from fellow histonetters.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 




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