[Histonet] FW: How to remove DAB to restain with DAB

[Amos Brooks]

Hi,
    There is not any practical way to remove the DAB. I think I read about
boiling it in a salt (of some sort) solution removing the DAB. Honestly I
never bothered trying it as you need to ask yourself "What is this doing to
my target antigen?". You should consider just restaining it with the DAB in
place using either Alkaline Phosphatase and Fast Red or using a fluorescent
detection if the DAB is interfering with the signal.

Amos


Message: 2
Date: Thu, 19 May 2011 11:55:14 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] FW: How to remove DAB to restain with DAB
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Hello,

I would like to repeat my DAB staining on the same slide with DAB I run
before.
I know that "acid alcohol" will remove hematoxylin but how to remove DAB?
I appreciate to any suggestion.

Thanks in advance,
Naira