[Histonet] FW: How to remove DAB to restain with DAB

[Patsy Ruegg]

I have removed DAB by using the probe cleaning reagents from the Dako
Autostainer but Amos is right this destroys the antigen site so it is of no
use.  If you really only have that one section I guess you might be able to
use it as an H&E.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Friday, May 20, 2011 2:25 PM
To: NMargaryan <@t> childrensmemorial.org; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: How to remove DAB to restain with DAB

Hi,
    There is not any practical way to remove the DAB. I think I read about
boiling it in a salt (of some sort) solution removing the DAB. Honestly I
never bothered trying it as you need to ask yourself "What is this doing to
my target antigen?". You should consider just restaining it with the DAB in
place using either Alkaline Phosphatase and Fast Red or using a fluorescent
detection if the DAB is interfering with the signal.

Amos


Message: 2
Date: Thu, 19 May 2011 11:55:14 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] FW: How to remove DAB to restain with DAB
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    <histonet-request <@t> lists.utsouthwestern.edu>
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    <histonet <@t> lists.utsouthwestern.edu>
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Hello,

I would like to repeat my DAB staining on the same slide with DAB I run
before.
I know that "acid alcohol" will remove hematoxylin but how to remove DAB?
I appreciate to any suggestion.

Thanks in advance,
Naira
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