[Histonet] IHC pos. & neg. control question

[Jean-Martin Lapointe]

I can certainly agree with that. Whether it’s the patient’s sample or the known positive control that are stained without primary, either would fulfill the purpose of detecting non-specific positive staining. 

Jean-Martin

 

 

From: Angela Bitting [mailto:akbitting <@t> geisinger.edu] 
Sent: Thursday, May 19, 2011 4:50 PM
To: Jean-Martin Lapointe; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question

 

I agree with the majority. A  patient slide stained without primary antibody, and a patient section with primary antibody and  a known positive control tissue, on the same slide when possible, is sufficient. I do agree, however, that the use of a negative tissue control is important in your initial antibody optimization and validation.

 

Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

 

email. Thank you.